Distinct cavemoviruses interact synergistically with sweet potato chlorotic stunt virus (genus Crinivirus) in cultivated sweet potato
Distinct cavemoviruses interact synergistically with sweet potato chlorotic stunt virus (genus Crinivirus) in cultivated sweet potato
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DOI：
10.1099/vir.0.029975-0
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发表时间：
2011-05-01
影响因子：
3.8
通讯作者：
Kreuze, Jan F.
中科院分区：
文献类型：
--
作者：
Cuellar, Wilmer J.;De Souza, Joao;Kreuze, Jan F.
分离到两种血清学上不相关的甘薯病毒，它们引起指示植物Ipomoea setosa的脉清症状，并对其基因组进行了测序。它们与甘薯中的无病毒感染有关，但当这些病毒与甘薯褪绿矮化病毒（SPCSV）共感染时，观察到明显的脉清症状和更高的病毒滴度，SPCSV是一种分布于世界各地的病毒，是该作物中严重病毒病的媒介。分子特征和系统发育分析显示，总体核苷酸的一致性为47.6%，并安排的运动蛋白和外壳蛋白结构域的成员属Cavemovirus，在家庭花椰菜花叶病毒。我们在东非、中美洲和加勒比海岛屿的栽培甘薯中检测到了这两种病毒，但在南美洲的样品中没有检测到。其中一种病毒的特征表现出类似的基因组组织，并形成了系统发育亚系，烟草脉清病毒（TVCV），进一步支持先前提出的TVCV分离，相关的病毒序列，到一个新的caulimovirid属。考虑到它们的地理分布和以前报道的类似但尚未鉴定的病毒，甘薯空洞病毒可能比以前认为的更经常与SPCSV共同发生，因此它们可能导致甘薯中混合病毒感染引起的广泛产量损失和品种下降。
Two serologically unrelated sweet potato viruses causing symptoms of vein clearing in the indicator plant lpomoea setosa were isolated and their genomes have been sequenced. They are associated with symptomless infections in sweet potato but distinct vein-clearing symptoms and higher virus titres were observed when these viruses co-infected with sweet potato chlorotic stunt virus (SPCSV), a virus that is distributed worldwide and is a mediator of severe virus diseases in this crop. Molecular characterization and phylogenetic analysis revealed an overall nucleotide identity of 47.6% and an arrangement of the movement protein and coat protein domains characteristic of members of the genus Cavemovirus, in the family Caulimoviridae. We detected both cavemoviruses in cultivated sweet potato from East Africa, Central America and the Caribbean islands, but not in samples from South America. One of the viruses characterized showed a similar genome organization as, and formed a phylogenetic sublineage with, tobacco vein clearing virus (TVCV), giving further support to the previously suggested separation of TVCV, and related viral sequences, into a new caulimovirid genus. Given their geographical distribution and previous reports of similar but yet unidentified viruses, sweet potato cavemoviruses may co-occur with SPCSV more often than previously thought and they could therefore contribute to the extensive yield losses and cultivar decline caused by mixed viral infections in sweet potato.