A collagen-binding mimetic of neural cell adhesion molecule.
A collagen-binding mimetic of neural cell adhesion molecule.
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DOI：
10.1021/bc700470v
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发表时间：
2008-05
影响因子：
4.7
通讯作者：
H. Miyazaki;Koichi Kato;Y. Teramura;H. Iwata
中科院分区：
文献类型：
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作者：
H. Miyazaki;Koichi Kato;Y. Teramura;H. Iwata
利用重组DNA技术合成了一种由神经细胞粘附分子衍生的细胞粘附肽和胶原结合结构域组成的嵌合蛋白。在这里，我们证明了嵌合蛋白结合I型胶原蛋白，促进海马神经元的粘附和轴突延伸。这些结果表明，嵌合蛋白质有潜力提供微环境，神经元粘附和生存在基于胶原的基质中用于基于细胞的治疗中枢神经系统疾病。
A chimeric protein consisting of a cell-adhesive peptide derived from a neural cell adhesion molecule and a collagen-binding domain was synthesized using recombinant DNA technology. Here, we demonstrate that the chimeric protein binds to type I collagen and promotes the adhesion and neurite extension of hippocampus neurons. These results suggest that the chimeric protein has potential to provide microenvironments for neurons to adhere and survive in collagen-based matrices for use in cell-based therapies for central nervous disorders.