Side population cells in human and mouse epidermis lack stem cell characteristics.
Side population cells in human and mouse epidermis lack stem cell characteristics.
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DOI：
10.1016/j.yexcr.2003.11.032
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发表时间：
2004-04
影响因子：
3.7
通讯作者：
Charlotte Triel;M. Vestergaard;L. Bolund;T. Jensen;U. Jensen
中科院分区：
文献类型：
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作者：
Charlotte Triel;M. Vestergaard;L. Bolund;T. Jensen;U. Jensen
在许多组织中发现了排除Hoechst 33342的细胞，这些细胞的共同点是通过流式荧光分析法分析时的特征谱（sp，侧群）。由于sp细胞在某些情况下起多能干细胞的作用，我们研究了表皮是否含有sp细胞（Esp细胞）以及这些细胞是否是表皮干细胞。我们发现，小鼠和人类表皮含有sp细胞，为了确定这些细胞的起源，我们测试了几个标记基因的表达。我们发现Esp细胞构成小鼠表皮α6整合素阳性基底细胞的一个亚群。它们对sca-1呈阳性，对MHC II类和Flk 1呈阴性。它们与标记保留群体不同，而是小鼠表皮中的循环细胞。sca-1阳性的角质形成细胞位于小鼠毛囊的含有干细胞的隆突区之外。对44个人类皮肤样本进行了分析，发现Esp细胞的频率范围为0.01%至5.39%，与年龄和身体部位无关。人Esp细胞不表达特别高水平的β1整合素。然而，他们表达的半转运ABCG 2，我们确定了高表达的汗腺分泌管上皮细胞，而低表达被发现在基底层的表皮这一标志物。
Cells that exclude Hoechst 33342 have been found in many tissues, and common for these cells is a characteristic profile when analysed by flow fluorimetry (sp, side population). Since sp cells in some cases function as multipotent stem cells, we investigated whether the epidermis contains sp cells (Esp cells) and whether these cells were epidermal stem cells. We show that mouse and human epidermis contain sp cells, and, to identify the origin of these cells, we tested the expression of several marker genes. We find that Esp cells constitute a subpopulation of the α6 integrin-positive basal cells of the mouse epidermis. They are positive for sca-1 and negative for MHC class II and Flk1. They are not identical to the label-retaining population but are cycling cells in the mouse epidermis. Keratinocytes positive for sca-1 are located outside the stem cell containing bulge area of the mouse hair follicle. Forty-four human skin samples were analysed, and Esp cells were found at frequencies ranging from 0.01% to 5.39%, independently of age and body site. Human Esp cells did not express particular high levels of β1 integrin. However, they expressed the half transporter ABCG2 and we identified high expression of this marker in the secretory duct epithelium of the sweat glands whereas low expression was found in the basal layer of the epidermis.