Transcriptional profiling of the early stages of germination in Candida albicans by real-time RT-PCR
Transcriptional profiling of the early stages of germination in Candida albicans by real-time RT-PCR
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DOI：
10.1016/j.femsyr.2004.08.004
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发表时间：
2004-12-01
影响因子：
3.2
通讯作者：
Chibana, H
中科院分区：
文献类型：
--
作者：
Toyoda, M;Cho, T;Chibana, H
通过使用实时RT-PCR，我们分析了CGR 1，CaMSI 3，EFG 1，NRG 1和TUP 1在白色念珠菌菌株JCM 9061和CAI 4中的表达，在几种条件下，包括诱导形态转变，热休克和钙抑制剂处理。CaMSI 3的表达在这些生长条件下发生变化，但在热休克。在JCM 9061中，CGR 1的表达在菌丝生长的早期阶段增加，而在热激过程中表达是应变依赖性的。EFG 1和NRG 1在菌丝诱导条件和热休克条件下表达相似。TUP 1的表达与EFG 1或NRG 1的表达略有不同。(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
By using real-time RT-PCR, we profiled the expression of CGR1, CaMSI3, EFG1, NRG1, and TUP1 in Candida albicans strains JCM9061 and CAI4 under several conditions, including induction of morphological transition, heat shock, and treatment with calcium inhibitors. Expression of CaMSI3 changed under these growth conditions except during heat shock. CGR1 expression increased during the early stages of hyphal growth in JCM9061, while expression was strain-dependent during heat shock. Both EFG1 and NRG1 were similarly expressed under hypha-inducing conditions and heat shock. Expression of TUP1 was slightly different from the expression of EFG1 or NRG1. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.