Prophylactic and therapeutic efficacy of the epitope vaccine CTB-UA against Helicobacter pylori infection in a BALB/c mice model
Prophylactic and therapeutic efficacy of the epitope vaccine CTB-UA against Helicobacter pylori infection in a BALB/c mice model
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表位疫苗CTB-UA对BALB/c小鼠模型幽门螺杆菌感染的预防和治疗效果
DOI：
10.1007/s00253-012-4122-0
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发表时间：
2012-09-01
影响因子：
5
通讯作者：
Xi, Tao
中科院分区：
文献类型：
--
作者：
Guo, Le;Liu, Kunmei;Xi, Tao
基于幽门螺杆菌尿素酶的抗原表位疫苗是预防和治疗幽门螺杆菌感染的一种有前途的选择。幽门感染本课题组在前期的研究中，利用霍乱毒素B亚单位（CT B）作为粘膜佐剂，结合霍乱弧菌UreA抗原表位（UreA），构建了霍乱毒素UreA抗原表位疫苗CTB-UA。pylori尿素酶A亚单位（UreA）。该疫苗具有良好的免疫原性和免疫反应性，能诱导产生特异性中和抗体，对H.幽门螺杆菌尿素酶。本研究在BALB/c小鼠模型中评价了表位疫苗CTB-UA的预防和治疗效果。实验结果表明，CTB-UA预防性或治疗性口服免疫可显著降低H。pylori定植与用PBS口服免疫相比。结果还显示，保护与抗原特异性IgG、伊加和粘膜分泌型伊加抗体应答相关。CTB-UA可能是一种有希望的控制H.幽门感染
Epitope vaccine based on the enzyme urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. pylori infection. In our previous study, the epitope vaccine CTB-UA, which was composed of the mucosal adjuvant cholera toxin B subunit (CTB) and an epitope (UreA₁₈₃₋₂₀₃) from the H. pylori urease A subunit (UreA) was constructed. This particular vaccine was shown to have good immunogenicity and immunoreactivity and could induce specific neutralizing antibodies, which exhibited effectively inhibitory effects on the enzymatic activity of H. pylori urease. In this study, the prophylactic and therapeutic efficacy of the epitope vaccine CTB-UA was evaluated in a BALB/c mice model. The experimental results indicated that oral prophylactic or therapeutic immunization with CTB-UA significantly decreased H. pylori colonization compared with oral immunization with PBS. The results also revealed that the protection was correlated with antigen-specific IgG, IgA, and mucosal secretory IgA antibody responses. CTB-UA may be a promising vaccine candidate for the control of H. pylori infection.