Disintegration of layer-by-layer assemblies composed of 2-iminobiotin-labeled poly(ethyleneimine) and avidin
Disintegration of layer-by-layer assemblies composed of 2-iminobiotin-labeled poly(ethyleneimine) and avidin
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DOI：
10.1021/bm0495856
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发表时间：
2005-01-01
影响因子：
6.2
通讯作者：
Anzai, J
中科院分区：
文献类型：
--
作者：
Inoue, H;Sato, K;Anzai, J
制备了由亲和素和2-亚氨基生物素标记的聚乙烯亚胺（ib-PEI）组成的层层膜，并研究了其对环境pH和生物素的敏感性。抗生物素蛋白/ib-PEI多层组装体在pH 8-12下是稳定的，而组装体在pH 5-6下由于质子化亚氨基生物素残基对抗生物素蛋白的低亲和力而分解。在溶液中加入生物素和类似物后，由于生物素或类似物优先结合到抗生物素蛋白的结合位点，抗生物素蛋白/ib-PEI组装体可以分解。通过改变刺激剂的种类（生物素或类似物）及其浓度，任意控制分解速率。加入生物素或脱硫生物素后，抗生物素蛋白/ib-PEI组装体迅速分解，而加入硫辛酸或2-（4 '-羟基苯偶氮）苯甲酸后，分解速率相当慢。本系统可用于构建可释放药物或其他功能分子的刺激敏感装置。
A layer-by-layer thin film composed of avidin and 2-iminobiotin-labeled poly(ethyleneimine) (ib-PEI) was prepared and their sensitivity to the environmental pH and biotin was studied. The avidin/ib-PEI multilayer assemblies were stable at pH 8-12, whereas the assemblies were decomposed at pH 5-6 due to the low affinity of the protonated iminobiotin residue to avidin. The avidin/ib-PEI assemblies can be disintegrated upon addition of biotin and analogues in the solution as a result of the preferential binding of biotin or analogues to the binding site of avidin. The decomposition rate was arbitrarily controlled by changing the type of stimulant (biotin or analogues) and its concentration. The avidin/ib-PEI assemblies were disintegrated rapidly by the addition of biotin or desthiobiotin, whereas the rate of decomposition was rather slow upon addition of lipoic acid or 2-(4'-bydroxyphenylazo)benzoic acid. The present system may be useful for constructing the stimuli-sensitive devices that can release drug or other functional molecules.