Poly(N-isopropylacrylamide)-Grafted Polydimethylsiloxane Substrate for Controlling Cell Adhesion and Detachment by Dual Stimulation of Temperature and Mechanical Stress
Poly(N-isopropylacrylamide)-Grafted Polydimethylsiloxane Substrate for Controlling Cell Adhesion and Detachment by Dual Stimulation of Temperature and Mechanical Stress
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DOI：
10.1021/acs.biomac.8b00992
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发表时间：
2018-10-01
影响因子：
6.2
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Akiyama, Yoshikatsu;Matsuyama, Miki;Okano, Teruo
制备了由聚（N-异丙基丙烯酰胺）（PI-PAAm）凝胶接枝的聚二甲基硅氧烷（PIPAAm-PDMS）组成的可拉伸温度响应性细胞培养表面，以证明温度和机械应力的双重刺激广泛地改变了接枝聚合物厚度、表面润湿性和细胞脱离行为。PIPAAm-PDMS表面在低于和高于下临界溶解温度时分别为亲水性和疏水性，这归因于PIPAAm链的相转变。当施加单轴拉伸时，接枝的PIPAAm凝胶表面被调制为更疏水，如通过接触角的增加所示。原子力显微镜观察发现，单轴拉伸使接枝凝胶层变薄，并使接枝PIPAAm凝胶的纳米级聚集体变形，这意味着PIPAAm链的延伸。在37 ℃下，拉伸的PIPAAm-PDMS变得比未拉伸的PIPAAm-PDMS更具有细胞粘附性。此外，双重刺激，收缩已经拉伸的PIPAAm-PDMS和降低温度，诱导更快的细胞脱离比只有温度的变化。类似地，在与温度或机械应力变化的单次刺激相比时，双重刺激加速了细胞片脱离和收获。这种新的可拉伸和温度响应的培养表面可以通过适当地组合每种刺激来容易地调节表面特性以适应不同的细胞片层，并且能够制造各种物种的细胞片层。
Stretchable temperature-responsive cell culture surfaces composed of poly(N-isopropylacrylamide) (PI-PAAm) gel-grafted polydimethylsiloxane (PIPAAm-PDMS) were prepared to demonstrate that dual stimulation of temperature and mechanical stress extensively altered graft polymer thickness, surface wettability, and cell detachment behavior. The PIPAAm-PDMS surface was hydrophilic and hydrophobic below and above the lower critical solution temperature, respectively, which was ascribed to the phase transition of PIPAAm chains. When uniaxial stretching was applied, the grafted PIPAAm gel surface was modulated to be more hydrophobic as shown by an increase in the contact angle. Atomic force microscopy observation revealed that uniaxial stretching made the grafted gel layer thinner and deformed the nanoscale aggregates of the grafted PIPAAm gel, implying extension of the PIPAAm chains. The stretched PIPAAm-PDMS became more cell adhesive than the unstretched PIPAAm-PDMS at 37 degrees C. Furthermore, dual stimulation, shrinking the already stretched PIPAAm-PDMS and decreasing the temperature, induced more rapid cell detachment than only a change in temperature did. Similarly, upon comparison with a single stimulation of a change in temperature or mechanical stress, dual stimulation accelerated cell sheet detachment and harvesting. This new stretchable and temperature-responsive culture surface can easily adjust the surface property to a different cell adhesiveness by appropriately combining each stimulus and enable the fabrication of cell sheets of various species.