Ultrasonic-assisted extraction of polysaccharides from Auricularia auricula and effects of its acid hydrolysate on the biological function of Caenorhabditis elegans
Ultrasonic-assisted extraction of polysaccharides from Auricularia auricula and effects of its acid hydrolysate on the biological function of Caenorhabditis elegans
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DOI：
10.1016/j.ijbiomac.2020.11.160
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发表时间：
2021-01-15
影响因子：
8.2
通讯作者：
Zhang, Yongjun
中科院分区：
文献类型：
--
作者：
Gu, Jingyi;Li, Qiaowei;Zhang, Yongjun
本研究以秀丽隐杆线虫为模型生物，采用Box-Behnken设计和酸水解的最佳提取条件，对黑木耳多糖制备的AAPs-H的体内抗氧化能力及其可能的机制进行了研究。研究了AAPs-H对秀丽隐杆线虫运动行为、寿命、抗氧化相关酶活性及抗氧化水平的影响。此外，还讨论了AAPs-H上调秀丽隐杆线虫抗氧化相关基因skn-1、sod-3和sir-2.1表达的潜力。aap - h对百草枯致伤具有极显著的保护作用，能显著提高虫体u型转弯频率（p < 0.01），延长虫体寿命，提高抗氧化系统GR 63.96% （p < 0.05）、GSH- px 71.16% （p < 0.01）、SOD 78.65% （p < 0.01）、CAT 98.52% (p < 0.01)，提高GSH水平28.12% (p < 0.05)，降低MDA水平39.29% （p < 0.01）。qRT-PCR结果显示，当AAPs-H浓度为0.4 mg/mL时，AAPs-H对野生型秀丽隐杆线虫中skn-1、sod-1、sod-2、sod-3和sir-2.1 mRNA的表达量上调了1.6倍（p< 0.05或p< 0.01）。我们的研究证明AAPs-H可以改善秀丽隐杆线虫的抗氧化防御系统，上调氧化应激相关基因，从而预防应激损伤。(C) 2020年Elsevier B.V.出版
The present study was designed to explore the in vivo-antioxidant capacity and the probable mechanism of AAPs-H, prepared from Auricularia auricula polysaccharides with the optimal extraction conditions by Box-Behnken design and acid hydrolysis, using Caenorhabditis elegans as a model organism. The effects of AAPs-H on the locomotion behavior, life span, antioxidant-related enzymes activities, and antioxidants levels in C. elegans were studied. Furthermore, the potentials of AAPs-H in up-regulating the expression of antioxidant-related genes in C. elegans, such as skn-1, sod-3 and sir-2.1, were also discussed. AAPs-H demonstrated a highly significant protective effect against the damage caused by paraquat, could significantly increase U-Turn frequency of worms (p < 0.01), extend their lifespan, enhance antioxidant systems including GR by 63.96% (p < 0.05), GSH-Px by 71.16% (p < 0.01), SOD by 78.65% (p < 0.01) and CAT by 98.52% (p < 0.01), increase the level of GSH by 28.12% (p < 0.05), and decrease the level of MDA by 39.29% (p < 0.01). The qRT-PCR results showed that AAPs-H could up regulate mRNA expression levels of skn-1, sod-1, sod-2, sod-3 and sir-2.1 in wild-type C. elegans (>1.6 fold) when treated with the concentration of 0.4 mg/mL (p< 0.05 or p < 0.01). Our studies provide evidence that AAPs-H improves antioxidant defense system, and up-regulation of oxidative stress related genes for prevention of stress damage in C. elegans. (C) 2020 Published by Elsevier B.V.