Neuronal organization in long term cultures of the spinal cord of the fetal mouse
Neuronal organization in long term cultures of the spinal cord of the fetal mouse
复制标题
胎鼠脊髓长期培养中的神经元组织
DOI：
10.1002/cne.901320302
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发表时间：
1968
影响因子：
2.5
通讯作者：
M. Bornstein
中科院分区：
文献类型：
--
作者：
H. Sobkowicz;R. W. Guillery;M. Bornstein
取14-16日龄胎鼠脊髓组织块，体外培养18-54天。将它们固定并用甲酚紫或Holmes方法染色。在大多数外植体中，细胞和纤维结构的基本模式是可识别的。在许多方面，在外植体中看到的模式类似于移植时的脊髓结构，并且两者中存在一些相同的细胞群。大多数外植体类似于脊髓的横切面。腹侧连合和背侧及腹侧神经根很容易辨认。此外，在外植体的周围形成两个特征性的圆形纤维带。
Explants of spinal cord from 14–16 day old fetal mice were cultured for 18–54 days in vitro. They were fixed and stained with cresyl violet or the Holmes method. In most explants a basic pattern of cell and fiber architectonics was recognizable. In many aspects the pattern seen in the explants resembles the structure of the spinal cord at the time of explantation, and some of the same cell groups are present in both. Most explants resmble a transverse section of spinal cord. A ventral commissure and dorsal and ventral roots are readily identified. In addition, two characteristic circular bands of fibers form around the periphery of the explants.