BIOCHEMICAL TOPOLOGY - APPLICATIONS TO DNA RECOMBINATION AND REPLICATION
BIOCHEMICAL TOPOLOGY - APPLICATIONS TO DNA RECOMBINATION AND REPLICATION
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DOI：
10.1126/science.3010458
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发表时间：
1986-05-23
期刊：
影响因子：
56.9
通讯作者：
COZZARELLI, NR
中科院分区：
文献类型：
--
作者：
WASSERMAN, SA;COZZARELLI, NR
DNA重排的过程，如重组或复制，常常产生无限数量的可区分的索链烷或结的不同子集。使用凝胶电泳和电子显微镜分析这些拓扑异构体，使人们有可能推断出的物理和几何特征的DNA结构和反应机制，否则实验无法访问。定量以及定性表征是可能的任何途径，其中的命运的环状DNA可以遵循。历史，理论和技术进行了审查，并从最近的研究中提出了说明性的例子。
Processes of DNA rearrangement such as recombination or replication frequently have as products different subsets of the limitless number of distinguishable catenanes or knots. The use of gel electrophoresis and electron microscopy for analysis of these topological isomers has made it possible to deduce physical and geometric features of DNA structure and reaction mechanisms that are otherwise experimentally inaccessible. Quantitative as well as qualitative characterization is possible for any pathway in which the fate of a circular DNA can be followed. The history, theory, and techniques are reviewed and illustrative examples from recent studies are presented.