Microbial conversion of dihydrooxoisophorone (DOIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) by thermophilic bacteria
Microbial conversion of dihydrooxoisophorone (DOIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) by thermophilic bacteria
复制标题
嗜热细菌将二氢氧代异佛尔酮 (DOIP) 转化为 4-羟基-2,2,6-三甲基环己酮 (4-HTMCH)
DOI：
10.1016/0168-1656(89)90081-3
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发表时间：
1989
影响因子：
4.1
通讯作者：
I. Karube
中科院分区：
文献类型：
--
作者：
K. Nishii;K. Sode;I. Karube
从土壤中分离的一种嗜热菌可将二氢氧异虫酮（DOIP, 2,2,6-三甲基-1,4-环己二酮）转化为4-羟基-2,2,6-三甲基环己酮（4-HTMCH）。鉴定该嗜热菌为嗜热脂肪芽孢杆菌。4-HTMCH有四种异构体：顺式-(4R, 6S)-、顺式-(4S, 6R)-、反式-(4R, 6R)-和反式-(4S, 6S)-。其数量比为68:25:5:2。在气升式反应器中，稀释率为0.07 h - 1，间歇培养20 h后，DOIP转化率为75%，连续培养为73%。在气升反应器中连续培养4-HTMCH的最大产率为200 mg l−1h−1，稀释率为0.12 h−1。这种微生物转化与细胞生长密切相关。研究了DOIP转化与细胞生长的关系。
Dihydrooxoisophorone (DOIP, 2,2,6-trimethyl-1,4-cyclohexanedione) was converted to 4-hydroxy-2,2,6-trimethylcyclohexanone (4-HTMCH) by a thermophile isolated from soil. The thermophile was identified asBacillus stearothermophilus. 4-HTMCH has four isomers:cis-(4R, 6S)-,cis-(4S, 6R)-,trans-(4R, 6R)- andtrans-(4S, 6S)-. Their quantitative ratio was 68:25:5:2. The extent of DOIP conversion was 75% in batch culture after 20 h cultivation and 73% in continuous culture at a dilution rate of 0.07 h−1in an air-lift reactor. The maximum production rate of 4-HTMCH in continuous culture in an air-lift reactor was 200 mg l−1h−1at a dilution rate of 0.12 h−1. This microbial conversion was closely associated with cell growth. The relationship between conversion of DOIP and cell growth was also investigated.