SEQUENCE-ANALYSIS AND COMPLEMENTATION STUDIES OF THE ARGJ GENE ENCODING ORNITHINE ACETYLTRANSFERASE FROM NEISSERIA-GONORRHOEAE
SEQUENCE-ANALYSIS AND COMPLEMENTATION STUDIES OF THE ARGJ GENE ENCODING ORNITHINE ACETYLTRANSFERASE FROM NEISSERIA-GONORRHOEAE
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DOI：
10.1128/jb.174.8.2694-2701.1992
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发表时间：
1992-04-01
影响因子：
3.2
通讯作者：
MULKS, MH
中科院分区：
文献类型：
--
作者：
MARTIN, PR;MULKS, MH
淋球菌临床分离株经常缺乏精氨酸生物合成。 这些营养缺陷型通常在精氨酸生物合成途径的第五步（乙酰鸟氨酸转化为鸟氨酸）中存在缺陷。 该反应由鸟氨酸乙酰转移酶催化，该酶是argJ基因的产物。 我们克隆并测序了淋球菌argJ基因，发现它包含一个1，218个核苷酸的开放阅读框架，编码一个推导的M（r）为42，879的肽。 这一预测的大小得到了微细胞分析的支持。 该基因能够互补大肠杆菌argE和argA突变，并转化N.淋病为Arg+。 Southern杂交能够检测到与铜绿假单胞菌基因组DNA中淋球菌argJ基因特异性杂交的条带，但不能检测到与大肠杆菌基因组DNA中淋球菌argJ基因特异性杂交的条带。这一结果反映了这些生物体中精氨酸生物合成途径的不同性质。
Clinical isolates of Neisseria gonorrhoeae frequently are deficient in arginine biosynthesis. These auxotrophs often have defects in the fifth step of the arginine biosynthetic pathway, the conversion of acetylornithine to ornithine. This reaction is catalyzed by the enzyme ornithine acetyltransferase, which is product of the argJ gene. We have cloned and sequenced the gonococcal argJ gene and found that it contains an open reading frame of 1,218 nucleotides and encodes a peptide with a deduced M(r) of 42,879. This predicted size was supported by minicell analysis. This gene was capable of complementing both Escherichia coli argE and argA mutations and of transforming an ArgJ- strain of N. gonorrhoeae to Arg+. Southern blots were able to detect bands that specifically hybridized to the gonococcal argJ gene in genomic DNA from Pseudomonas aeruginosa but not E. coli, a result that reflects the divergent nature of the arginine biosynthetic pathway in these organisms.