Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases
Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases
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DOI：
10.1002/ptr.1377
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发表时间：
2003-09-01
影响因子：
7.2
通讯作者：
Luna-Herrera, J
中科院分区：
文献类型：
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作者：
Jimenez-Arellanes, A;Meckes, M;Luna-Herrera, J
耐多药结核分枝杆菌（MDR-TB）的增加要求寻找替代的抗分枝杆菌药物。本研究的目的是评估墨西哥传统医学中用于治疗呼吸道疾病的植物对耐多药结核病的活性。在50 - 200 μ g/mL的浓度下筛选一组22株植物的抗结核分枝杆菌H37 Rv和鸟分枝杆菌的活性。用阿利兰染料微量比色法测定其抗分枝杆菌作用。水提取物均无抗分枝杆菌活性。沙蒿、山慈菇、马缨丹、刺柏和锦葵的正己烷提取物和沙蒿、刺柏的甲醇提取物对结核分枝杆菌的生长有抑制作用。刺柏正己烷提取物和锦葵甲醇提取物对鸟分枝杆菌有抑制作用。活性提取物针对结核分枝杆菌H37 Rv的单抗性变体（异烟肼、利福平、链霉素和乙胺丁醇抗性）进行测试，并且马缨丹的己烷提取物显示出最佳活性。马缨丹己烷提取物对一组耐多药结核病临床分离株也有活性。相反，它不抑制非结核分枝杆菌的生长。通过柱色谱法对马缨丹的己烷提取物进行分级分离，其中一个级分（FVI）在浓度高达25 μ g/mL时抑制所有MDR-TB临床分离株的生长。这项研究支持了这样一个事实，即选择植物的民族植物学标准提高了发现具有抗分枝杆菌活性的物种的可能性，我们的研究结果指出，马缨丹作为抗耐多药结核病的潜在化合物的重要来源。版权所有（C）2003约翰威利父子有限公司。
The increase of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) demands the search for alternative antimycobacterial drugs. The aim of this study was to evaluate plants used in Mexican traditional medicine to treat respiratory diseases for activity against MDR-TB. A group of 22 plants was screened for activity against Mycobacterium tuberculosis H37Rv and Mycobacterium avium at concentrations from 50 to 200 mug/mL. The antimycobacterial effect was determined by a microcolorimetric assay with Alamar blue dye. None of the aqueous extracts had antimycobacterial activity. Hexane extracts from Artemisia ludoviciana, Chamaedora tepejilote, Lantana hispida, Juniperus communis and Malva parviflora, and methanol extracts from Artemisia ludoviciana and Juniperus communis inhibited the growth of Mycobacterium tuberculosis. Mycobacterium avium was inhibited by Juniperus communis hexane extract and by Malva parviflora methanol extract. The active extracts were tested against monoresistant variants of Mycobacterium tuberculosis H37Rv (isoniazid, rifampin, streptomycin and ethambutol resistant) and the hexane extract of Lantana hispida showed the best activity. Lantana hispida hexane extract was also active against a group of MDR-TB clinical isolates. In contrast, it did not inhibit the growth of non-tuberculous mycobacteria. The hexane extract of Lantana hispida was fractionated by column chromatography and one of its fractions (FVI) inhibited the growth of all the MDR-TB clinical isolates at concentrations up to 25 mug/mL. This study supports the fact that selecting plants by ethnobotanical criteria enhances the probability of finding species with activity against mycobacteria, and our results point to Lantana hispida as an important source of potential compounds against MDR-TB. Copyright (C) 2003 John Wiley Sons, Ltd.