Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila
Constitutive activation of toll-mediated antifungal defense in serpin-deficient Drosophila
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DOI：
10.1126/science.285.5435.1917
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发表时间：
1999-09-17
期刊：
影响因子：
56.9
通讯作者：
Reichhart, JM
中科院分区：
文献类型：
--
作者：
Levashina, EA;Langley, E;Reichhart, JM
果蝇的抗真菌防御是由Spaetzle/Toll/仙人掌基因盒控制的，在这里，编码血丝氨酸蛋白酶抑制物Spn43Ac基因的功能缺失突变导致了抗真菌多肽Drosmycin的结构性表达，这种作用是由Spaetzle和Toll基因产物介导的。Spn4Ac缺陷型果蝇在免疫攻击后不久，Spaetzle被蛋白水解酶裂解为其活性配基形式，裂解Spaetzle是结构性存在的。因此，Spn43Ac负性调节Toll信号通路，Toll在果蝇的寄主防御中不起模式识别受体的作用。
The antifungal defense of Drosophila is controlled by the spaetzle/Toll/cactus gene cassette, Here, a Loss-of-function mutation in the gene encoding a blood serine protease inhibitor, Spn43Ac, was shown to Lead to constitutive expression of the antifungal peptide drosomycin, and this effect was mediated by the spaetzle and Toll gene products. Spaetzle was cleaved by proteolytic enzymes to its active Ligand form shortly after immune challenge, and cleaved Spaetzle was constitutively present in Spn4Ac-deficient flies. Hence, Spn43Ac negatively regulates the Toll signaling pathway, and Toll does not function as a pattern recognition receptor in the Drosophila host defense.