DISTRIBUTION PATTERNS OF EXTRACELLULAR-MATRIX COMPONENTS AND ADHESION RECEPTORS ARE INTRICATELY MODULATED DURING 1ST TRIMESTER CYTOTROPHOBLAST DIFFERENTIATION ALONG THE INVASIVE PATHWAY, INVIVO
DISTRIBUTION PATTERNS OF EXTRACELLULAR-MATRIX COMPONENTS AND ADHESION RECEPTORS ARE INTRICATELY MODULATED DURING 1ST TRIMESTER CYTOTROPHOBLAST DIFFERENTIATION ALONG THE INVASIVE PATHWAY, INVIVO
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DOI：
10.1172/jci115565
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发表时间：
1992-01-01
影响因子：
15.9
通讯作者：
FISHER, SJ
中科院分区：
文献类型：
--
作者：
DAMSKY, CH;FITZGERALD, ML;FISHER, SJ
人类胚胎的发育取决于早期妊娠细胞滋养层干细胞分化和侵入子宫的能力。在这个过程中，侵袭性表型的瞬时表达是正常细胞滋养层分化的一部分。从形态学上讲，这一过程始于极化的绒毛细胞滋养层形成多层非极化细胞柱，并侵入子宫。使用免疫细胞化学，我们比较了绒毛、细胞柱和子宫壁中细胞滋养层上粘附受体和细胞外基质配体的存在。绒毛细胞滋养层，锚定到基底膜，染色的α-6和β-4整合素亚基和两个merosin和A-链含有层粘连蛋白。非极化的细胞滋养层主要表达α-5和β-1整合素亚基和富含纤连蛋白的基质。子宫壁中的细胞滋养层簇被α-1，α-5和β-1整合素染色，但不被大多数细胞外基质抗原染色，表明它们主要与母体细胞和基质相互作用。腱生蛋白染色仅限于细胞滋养层形态过渡部位的基质，表明腱生蛋白影响细胞滋养层分化。我们的研究结果表明，调节粘附分子的表达有助于收购的侵袭性表型细胞滋养层细胞，并提供了一个基础，研究病理条件下，滋养层浸润不足或过度发生，如先兆子痫或绒毛膜癌。
Development of the human embryo depends on the ability of first trimester cytotrophoblastic stem cells to differentiate and invade the uterus. In this process, transient expression of an invasive phenotype is part of normal cytotrophoblast differentiation. Morphologically, this process begins when polarized chorionic villus cytotrophoblasts form multilayered columns of nonpolarized cells, and invade the uterus. Using immunocytochemistry, we compared the presence of adhesion receptors and extracellular matrix ligands on cytotrophoblasts in villi, cell columns, and the uterine wall. Villus cytotrophoblasts, anchored to basement membrane, stained for alpha-6 and beta-4 integrin subunits and both merosin and A-chain-containing laminin. Nonpolarized cytotrophoblasts in columns expressed primarily alpha-5 and beta-1 integrin subunits and a fibronectin-rich matrix. Cytotrophoblast clusters in the uterine wall stained for alpha-1, alpha-5, and beta-1 integrins, but not for most extracellular matrix antigens, suggesting that they interact primarily with maternal cells and matrices. Tenascin staining was restricted to stroma at sites of transition in cytotrophoblast morphology, suggesting that tenascin influences cytotrophoblast differentiation. Our results suggest that regulation of adhesion molecule expression contributes to acquisition of an invasive phenotype by cytotrophoblasts and provide a foundation for studying pathological conditions in which insufficient or excessive trophoblast invasion occurs, such as preeclampsia or choriocarcinoma.