Characterization of ACE and ACE2 Expression within Different Organs of the NOD Mouse.
Characterization of ACE and ACE2 Expression within Different Organs of the NOD Mouse.
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DOI：
10.3390/ijms18030563
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发表时间：
2017-03-05
影响因子：
5.6
通讯作者：
Soler MJ
中科院分区：
文献类型：
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作者：
Roca-Ho H;Riera M;Palau V;Pascual J;Soler MJ
众所周知，肾素血管紧张素系统（RAS）在糖尿病、肾脏和心血管疾病等多种疾病中起着关键作用。它的阻断已被证明可以延缓糖尿病患者慢性肾脏疾病的进展和心血管损害。从这个意义上说，既然已经描述了局部RAS，本研究的目的是表征血管紧张素转换酶（ACE）和ACE2活性，以及蛋白质表达，在非肥胖糖尿病（NOD）小鼠模型的几个组织中。在糖尿病发病21天和40天后，分析小鼠血清和组织中ACE和ACE2酶活性和蛋白表达。检测不同组织的ACE和ACE2酶活性。它们的表达因所研究的组织而异。因此，在研究的组织中，ACE活性在肺部高表达，而ACE2活性在胰腺高表达。有趣的是，我们还观察到糖尿病主要上调血清、肺、心脏和肝脏中的ACE，上调血清、肝脏和胰腺中的ACE2。总之，我们发现糖尿病小鼠的血清和肺部ACE活性明显改变，提示有共同的调节。糖尿病小鼠循环内ACE2活性的增加可能归因于RAS的代偿机制。
Renin angiotensin system (RAS) is known to play a key role in several diseases such as diabetes, and renal and cardiovascular pathologies. Its blockade has been demonstrated to delay chronic kidney disease progression and cardiovascular damage in diabetic patients. In this sense, since local RAS has been described, the aim of this study is to characterize angiotensin converting enzyme (ACE) and ACE2 activities, as well as protein expression, in several tissues of the non-obese diabetic (NOD) mice model. After 21 or 40 days of diabetes onset, mouse serums and tissues were analyzed for ACE and ACE2 enzyme activities and protein expression. ACE and ACE2 enzyme activities were detected in different tissues. Their expressions vary depending on the studied tissue. Thus, whereas ACE activity was highly expressed in lungs, ACE2 activity was highly expressed in pancreas among the studied tissues. Interestingly, we also observed that diabetes up-regulates ACE mainly in serum, lung, heart, and liver, and ACE2 mainly in serum, liver, and pancreas. In conclusion, we found a marked serum and pulmonary alteration in ACE activity of diabetic mice, suggesting a common regulation. The increase of ACE2 activity within the circulation in diabetic mice may be ascribed to a compensatory mechanism of RAS.