A COMPARATIVE-ANALYSIS OF STREAMING POTENTIALS INVIVO AND INVITRO
A COMPARATIVE-ANALYSIS OF STREAMING POTENTIALS INVIVO AND INVITRO
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DOI：
10.1002/jor.1100100513
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发表时间：
1992-09-01
影响因子：
2.8
通讯作者：
COCHRAN, GV
中科院分区：
文献类型：
--
作者：
OTTER, MW;PALMIERI, VR;COCHRAN, GV
流动电位（SP）在体内测量在一个特定的网站上完整的皮质骨（犬胫骨）进行了比较，从相同的网站在体外的测量，测试作为一个切除的骨条浸泡在汉克的平衡盐溶液。在13个胫骨中，体外每个骨膜应变的SP幅度大于体内（在1 Hz时平均x6.5），但每个经皮质应变差的值相似。在体外，SP幅度更急剧上升到一个渐近值作为弯曲频率的函数比在体内的信号，可能是因为小管和/或哈弗系统的内部状态的差异。同样，SP响应阶跃负荷下降到零更慢，随着时间的推移，在体外比在体内。由于通过电解质和软组织的电分流，在初步测量中遇到的困难表明，在使用体内和体外SP测量外推到细胞水平的电场强度时需要谨慎。
Streaming potentials (SPs) measured in vivo at a specific site on intact cortical bone (canine tibia) have been compared with measurements from the same site in vitro, tested as an excised bone strip soaked in Hank's balanced salt solution. The amplitude of SPs per periosteal strain in vitro was larger in 13 tibias than in vivo (by an average x6.5 at 1 Hz), but values per transcortical strain difference were similar. In vitro, SP magnitudes rose more sharply to an asymptotic value as a function of bending frequency than did in vivo signals, possibly because of a difference in the intemal state of canaliculi and/or Haversian systems. Similarly, SP response to step-loading decreased to zero more slowly with time in vitro than in vivo. Difficulties encountered in preliminary measurements due to electrical shunting through electrolyte and soft tissues suggest the need for caution in using both in vivo and in vitro SP measurements to extrapolate to electric field strengths on the cellular level.