Real-Time in Situ Visualizing of the Sequential Activation of Caspase Cascade Using a Multicolor Gold-Selenium Bonding Fluorescent Nanoprobe
Real-Time in Situ Visualizing of the Sequential Activation of Caspase Cascade Using a Multicolor Gold-Selenium Bonding Fluorescent Nanoprobe
复制标题
使用多色金硒键合荧光纳米探针实时原位可视化 Caspase 级联的顺序激活
DOI：
10.1021/acs.analchem.9b00452
复制
发表时间：
2019-05-07
影响因子：
7.4
通讯作者：
Tang, Bo
中科院分区：
文献类型：
--
作者：
Liu, Xiaojun;Song, Xiaoxiao;Tang, Bo
半胱天冬酶级联反应是一组非常重要的信号分子，在细胞凋亡中起着关键作用。实时监测信号通路中caspase的上下游激活关系，对于了解这些信号分子在各种疾病发生发展中的调控机制具有重要意义。本文基于金硒键合原理，研制了一种新型的纳米金硒荧光探针GNP-Se-Casp，用于细胞凋亡过程中caspase-（carp-）3、8和9的实时原位监测。在使用GNP-Se-Casp的由星形孢菌素诱导的凋亡HeLa细胞的实时荧光成像中，对应于casp-8和casp-9的荧光信号依次开启，随后出现casp-3的荧光，其可视化casp-3、casp-8和casp-9的上游和下游关系。因此，GNP-Se-Casp是实时原位监测肿瘤细胞凋亡过程中caspase级联激活的有效工具。这种设计策略很容易适用于其他信号分子的原位检测，特别是那些与上游和下游激活关系。
The caspase cascade is an ensemble of very important signaling molecules that plays a critical role in cell apoptosis. Real-time monitoring of the upstream and downstream activation relationships of the caspases in the signal pathway is of great significance for understanding the regulatory mechanisms of these signaling molecules in the development of various diseases. Herein, a multicolor fluorescent nanoprobe, GNP-Se-Casp, has been developed based on Au-Se bonding for real-time in situ monitoring caspase- (carp-) 3, 8, and 9 during cell apoptosis. In the real-time fluorescence imaging of apoptotic HeLa cells induced by staurosporine using GNP-Se-Casp, the fluorescence signals corresponding to casp-8 and casp-9 sequentially turn on, followed by the appearance of the fluorescence of casp-3, which visualizes the upstream and downstream relationships of casp-3,-8, and -9. Thus, GNP-Se-Casp is an effective tool for real-time in situ monitoring of caspase cascade activation in the apoptosis process of tumor cells. This design strategy is easily adaptable to in situ detection of other signal molecules, especially those with upstream and downstream activation relationships.