Ginkgolide B and bilobalide ameliorate neural cell apoptosis in α-synuclein aggregates
Ginkgolide B and bilobalide ameliorate neural cell apoptosis in α-synuclein aggregates
复制标题
银杏内酯 B 和白果内酯可改善 α-突触核蛋白聚集体中的神经细胞凋亡。
DOI：
10.1016/j.biopha.2017.10.050
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发表时间：
2017-12-01
影响因子：
7.5
通讯作者：
Hu, Gang
中科院分区：
文献类型：
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作者：
Hua, Jun;Yin, Nuo;Hu, Gang
聚集形式的α-突触核蛋白（α-Syn）的积累与帕金森病（PD）（一种慢性进行性神经退行性疾病）的发病机制相关。大量证据表明，银杏叶消费对PD后的运动活动有很好的影响。然而，这些机制强调了α-Syn诱导的细胞损伤，以及银杏内酯是否对这种损伤发挥神经保护作用尚不清楚。在这里，我们通过流式细胞术和蛋白质印迹分析表明，聚集的重组人α-Syn，而不是α-Syn单体，以细胞凋亡的方式触发了培养的人神经母细胞瘤细胞系SH-SY 5 Y的细胞损伤。此外，用银杏内酯B（GB）或白果内酯（BB）预处理保护SH-SY 5 Y细胞免受α-Syn诱导的细胞活力降低，并减少聚集的α-Syn刺激后的细胞凋亡。本研究首次发现聚集的α-Syn诱导细胞凋亡，而GB和BB可减弱聚集的α-Syn诱导的细胞凋亡，这为今后PD的治疗提供了新的思路。
The accumulation of aggregated forms of the alpha-Synuclein (alpha-Syn) is associated with the pathogenesis of Parkinson's disease (PD), a chronic progressive neurodegenerative disorder. Extensive evidences have shown the promising effects of Ginkgo biloba consumption on motor activity after PD. However, the mechanisms underline the alpha-Syn-induced cell damage and whether ginkgolides exert neuroprotection against this injury are unclear. Here we showed that aggregated recombinant human alpha-Syn, but not alpha-Syn monomers, triggered cell injury in cultured human neuroblastoma cell line SH-SY5Y in an apoptosis way by using flow cytometry and western blot assay. Moreover, pre-treatment with the Ginkgolide B (GB) or Bilobalide (BB) protected SH-SY5Y cells against alpha-Syn- induced cell viability decreases, and reduced cell apoptosis after aggregated alpha-Syn stimulation. Together, we firstly find that aggregated alpha-Syn induced cell apoptosis and GB and BB may attenuate aggregated alpha-Syn-induced cell apoptosis, which gives us an insight into the novel therapy for PD in future.