COMPARISON OF 3 REAGENTS FOR DETECTING INDOLE PRODUCTION BY ANAEROBIC-BACTERIA IN MICROTEST SYSTEMS
COMPARISON OF 3 REAGENTS FOR DETECTING INDOLE PRODUCTION BY ANAEROBIC-BACTERIA IN MICROTEST SYSTEMS
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DOI：
10.1128/jcm.18.3.609-613.1983
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发表时间：
1983-01-01
影响因子：
9.4
通讯作者：
DOWELL, VR
中科院分区：
文献类型：
--
作者：
LOMBARD, GL;DOWELL, VR
使用商用微量测试系统对用于检测吲哚的三种试剂（Kovac、Ehrlich 和对二甲氨基肉桂醛 (DMCA)）进行评估，以表征和鉴定厌氧菌。 DMCA试剂是3种试剂中最敏感的，它与449株各种产吲哚厌氧菌中的445株呈阳性反应。在微量测试系统中使用 DMCA 获得的结果与使用二甲苯萃取和艾利希试剂检测吲哚的传统管测试获得的结果之间有 99.6% 的一致性。当在孵育前用矿物油覆盖接种物时，艾利希试剂在 176 株吲哚阳性菌株中的 163 株（92.6%）中检测到吲哚。 Kovac 试剂是测试试剂中最不敏感的。当接种物上覆盖矿物油时，Kovac 试剂仅检测到 108 株吲哚阳性菌株中的 80 株（74.0%）。 DMCA 除了是检测吲哚最灵敏的试剂外，还可以检测某些梭菌产生的吲哚衍生物（粪臭素、3-吲哚丙酸和 3-吲哚丁酸）。
Three reagents for detecting indole, Kovac, Ehrlich and p-dimethylaminocinnamaldehyde (DMCA), were evaluated with commercial microtest systems for characterizing and identifying anaerobic bacteria. The DMCA reagent, the most sensitive of the 3 reagents, gave a positive reaction with 445 of 449 strains of various indole-producing anaerobic bacteria. There was 99.6% agreement between the results obtained with the DMCA in the microtest systems and results using the conventional tube test to detect indole by using xylene extraction and Ehrlich reagent. Ehrlich reagent detected indole in 163 of 176 (92.6%) indole-positive strains when the inoculum was overlaid with mineral oil before incubation. Kovac reagent was the least sensitive of the reagents tested. When the inoculum was overlaid with mineral oil, Kovac reagent detected only 80 of 108 (74.0%) of indole-positive strains. In addition to being the most sensitive reagent for detection indole, DMCA also allowed detection of indole derivatives (skatole, 3-indolepropionic acid and 3-indolebutyric acid) produced by some clostridia.