Spatio-temporal activity patterns of odor-induced synchronized potentials revealed by voltage-sensitive dye imaging and intracellular recording in the antennal lobe of the cockroach.
Spatio-temporal activity patterns of odor-induced synchronized potentials revealed by voltage-sensitive dye imaging and intracellular recording in the antennal lobe of the cockroach.
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DOI：
10.3389/fnsys.2012.00055
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发表时间：
2012
影响因子：
3
通讯作者：
Yokohari F
中科院分区：
文献类型：
--
作者：
Watanabe H;Ai H;Yokohari F
在动物中，气味的质量表示为肾小球的空间活动模式和初级嗅觉中心同步振荡信号的时间模式。通过光学成像的电压敏感染料（VSD）和细胞内记录从次级嗅觉中间神经元，我们研究了可能的神经相关的空间和时间的气味表示在初级嗅觉中心，触角叶（AL），美洲大蠊。电压敏感染料成像显示，所有使用的气味诱导的气味特定的时间模式的去极化电位在特定的组合前肾小球的AL。不同的气味诱发的去极化电位在时间上同步的肾小球，并被称为“同步电位”。这些观察结果表明，气味的质量是由肾小球同步电位的时空活动模式。我们还进行了细胞内记录和染色的次级嗅觉中间神经元，即投射神经元和局部中间神经元。我们分析了庚酸诱导的次级嗅觉中间神经元的动作电位的时间结构，同时从两个给定的神经元配对的细胞内记录。我们的研究结果表明，多个局部中间神经元同步响应嗅觉刺激。此外，所有染色的庚酸反应投射神经元表现出树突状arborizations在肾小球内的同步电位被诱发。由于已知多个局部中间神经元与蟑螂AL中每个肾小球中的投射神经元突触，因此从局部中间神经元到投射神经元的会聚输入似乎有助于同步电位的气味特异性时空活动模式。
In animals, odor qualities are represented as both spatial activity patterns of glomeruli and temporal patterns of synchronized oscillatory signals in the primary olfactory centers. By optical imaging of a voltage-sensitive dye (VSD) and intracellular recording from secondary olfactory interneurons, we examined possible neural correlates of the spatial and temporal odor representations in the primary olfactory center, the antennal lobe (AL), of the cockroach Periplaneta americana. Voltage-sensitive dye imaging revealed that all used odorants induced odor-specific temporal patterns of depolarizing potentials in specific combinations of anterior glomeruli of the AL. The depolarizing potentials evoked by different odorants were temporally synchronized across glomeruli and were termed “synchronized potentials.” These observations suggest that odor qualities are represented by spatio-temporal activity patterns of the synchronized potentials across glomeruli. We also performed intracellular recordings and stainings from secondary olfactory interneurons, namely projection neurons and local interneurons. We analyzed the temporal structures of enanthic acid-induced action potentials of secondary olfactory interneurons using simultaneous paired intracellular recording from two given neurons. Our results indicated that the multiple local interneurons synchronously fired in response to the olfactory stimulus. In addition, all stained enanthic acid-responsive projection neurons exhibited dendritic arborizations within the glomeruli where the synchronized potentials were evoked. Since multiple local interneurons are known to synapse to a projection neuron in each glomerulus in the cockroach AL, converging inputs from local interneurons to the projection neurons appear to contribute the odorant specific spatio-temporal activity patterns of the synchronized potentials.