Modulation of chlorophyll contents and anti-oxidant systems in two cucumber varieties under waterlogging stress and subsequent drainage
Modulation of chlorophyll contents and anti-oxidant systems in two cucumber varieties under waterlogging stress and subsequent drainage
复制标题
涝渍胁迫和后续排水下两个黄瓜品种叶绿素含量和抗氧化系统的调节
DOI：
10.1080/14620316.2011.11512770
复制
发表时间：
2011-01
影响因子：
1.9
通讯作者：
Chen, X. H.
中科院分区：
文献类型：
--
作者：
Qi, X. H.;Li, T.;Xu, Q.;Chen, X. H.
黄瓜（Cucumis sativus L.）在夏季很容易受到暴雨和随后的洪水的影响。以黄瓜耐涝系PW0832和耐涝敏感系PW0801为对照，研究了黄瓜耐涝的生理基础和抗涝恢复能力。叶绿素含量、抗氧化酶活性和脂质过氧化产物水平在涝渍条件下和恢复后的7 d内进行测定。两种基因型的耐受性差异取决于其抗氧化系统的早期激活和各自从涝渍胁迫中恢复的速度。超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性的升高在维持PW0832的生长和促进其从涝渍胁迫中恢复中发挥了重要作用。PW0801恢复能力低与脂质过氧化损伤、叶绿素含量降低、SOD和CAT活性降低有关。两种黄瓜基因型在涝渍胁迫下的过氧化物酶（POD）活性均低于非涝渍对照植株，表明黄瓜对涝渍胁迫的适应可能不依赖于POD活性。
Summary Cucumber (Cucumis sativus L.) is easily affected by heavy rain and subsequent periods of flooding during the Summer. In the present study, the physiological basis for the tolerance of cucumber plants to waterlogging and their ability to recover from this stress was investigated using two contrasting genotypes: the waterlogging-tolerant line, PW0832, and the waterlogging-sensitive line, PW0801. Chlorophyll contents, anti-oxidant enzyme activities, and levels of the products of lipid peroxidation were measured over 7 d under waterlogged conditions and for 7 d after recovery. The difference in tolerance level between the two genotypes was dependent on the early activation of their anti-oxidant systems and their respective rates of recovery from waterlogging stress. The elevated activities of superoxide dismutase (SOD) and catalase (CAT) played important roles in maintaining growth and facilitating the recovery of PW0832 from waterlogging stress. The low recovery capacity of PW0801 was associated with the damage caused by lipid peroxidation, a reduction in chlorophyll content, and decreases in SOD and CAT activities. The lower peroxidase (POD) activities in both cucumber genotypes under waterlogging stress compared with those in non-stressed control plants indicated that acclimatisation to waterlogging stress may not be dependent on POD activity.