On the mechanism of genetic recombination: electron microscopic observation of recombination intermediates.
On the mechanism of genetic recombination: electron microscopic observation of recombination intermediates.
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论基因重组的机制：重组中间体的电子显微镜观察。
DOI：
10.1073/pnas.73.9.3000
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发表时间：
1976
影响因子：
11.1
通讯作者：
D. Dressler
中科院分区：
文献类型：
--
作者：
H. Potter;D. Dressler
本文讨论重组中间体的性质。使用电子显微镜研究质粒大肠杆菌素 E1 的 DNA，我们观察到了 800 多个分子，这些分子似乎代表重组过程中的中间体。具体来说，在分离大肠菌素 DNA 并用限制酶 EcoRI 将其线性化后，我们发现交叉分子的大肠菌素 DNA 含量是正常大肠菌素 DNA 含量的两倍。这些形式由在 DNA 同源区域结合在一起的两个基因组长度的元件组成。这些分子可以从野生型和 Rec B-C 宿主细胞中回收，但不存在于从重组缺陷型 Rec A 细胞中分离的大肠菌素 DNA 形式中。我们将实验观察到的分子称为“chi 形式”，并相信它们代表霍利迪模型的重组中间体。
This paper deals with the nature of recombination intermediates. Using the electron microscope to study the DNA of the plasmid colicin E1, we have observed more than 800 molecules that appear to represent intermediates in the process of recombination. Specifically, after isolating colicin DNA and linearizing it with the restriction enzyme EcoRI, we find crossed molecules with twice the normal colicin DNA content. These forms consist of two genome-length elements held together at a region of DNA homology. The molecules can be recovered from wild type and Rec B-C host cells but are not present among the colicin DNA forms isolated from recombination-deficient Rec A cells. We have termed the experimentally observed molecules "chi forms" and believe that they represent the recombination intermediate of the Holliday model.