Increased proportion of nitric oxide synthase immunoreactive neurons in rat ileal myenteric ganglia after severe acute pancreatitis.
Increased proportion of nitric oxide synthase immunoreactive neurons in rat ileal myenteric ganglia after severe acute pancreatitis.
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DOI：
10.1186/1471-230x-11-127
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发表时间：
2011-11-23
影响因子：
2.4
通讯作者：
Hu Q
中科院分区：
文献类型：
--
作者：
Lin Z;Liu Y;Zheng Q;Hu Q
严重急性胰腺炎（SAP）仍然是一种潜在的危及生命的疾病。胃肠运动障碍如肠梗阻在每一个病例中都可见。到目前为止，胰腺炎诱发肠梗阻的机制在很大程度上是未知的。本研究的主要目的是观察胃肠道运动障碍大鼠回肠肌神经节一氧化氮合酶免疫反应（NOS-IR）神经元的变化，探讨胰腺炎致肠梗阻的潜在神经机制。20只大鼠随机分为假手术组和SAP组。采用逆行胆管胰管注射5%牛磺胆酸钠诱导SAP。通过腹部x线及肠行检查是否存在麻痹性肠梗阻和肠蠕动障碍。评估胰腺病理损害。采用双免疫标记法制备回肠肌神经节。观察NOS-IR神经元形态，计算NOS-IR神经元的百分率。按照Trizol试剂方案提取回肠总RNA。RT-PCR检测神经元NOS mRNA表达。SAP组小肠转运指数明显低于假手术组（29.21±3.68% vs 52.48±6.76%,P <0.01）。SAP组回肠肌间神经节NOS-IR神经元比例显著高于假手术组（37.5±12.28% vs 26.32±16.15%,P <0.01）。SAP组回肠nNOS mRNA表达量显著高于假手术组（1.02±0.10 vs 0.70±0.06,P < 0.01）。回肠肌间神经节NOS-IR神经元数量增加，nNOS mRNA表达增加，提示nNOS过表达可能是SAP大鼠胃肠运动障碍的神经机制之一。
Severe acute pancreatitis (SAP) remains a potentially life-threatening disease. Gastrointestinal motility disturbance such as intestinal ileus is seen in every case. By now, the mechanisms of pancreatitis-induced ileus are largely unknown. The main purpose of the present study was to observe changes of nitric oxide synthase-immunoreactive (NOS-IR) neurons in ileal myenteric ganglia in SAP rats with gastrointestinal dysmotility, trying to explore underlying nervous mechanisms of pancreatitis-induced ileus. Twenty Sprague Dawley rats were randomly divided into sham operated group and SAP group. SAP was induced by retrograde cholangiopancreatic duct injection of 5% sodium taurocholate. Abdominal X-ray and intestinal transit were performed to detect the existence of paralytic ileus and intestinal dysmotility. Pathological damage of pancreas was evaluated. Double-immunolabeling was employed for the whole-mount preparations of ileal myenteric ganglia. The morphology of NOS-IR neurons were observed and the percentage of NOS-IR neurons was calculated based on the total Hu-immunoreactive neurons. Total RNA of ileum was extracted according to Trizol reagent protocol. Neuronal NOS (nNOS) mRNA expression was evaluated by RT-PCR. The small intestinal transit index in the SAP group was significantly lower compared with the sham operated group (29.21 ± 3.68% vs 52.48 ± 6.76%, P <0.01). The percentage of NOS-IR neurons in ileal myenteric ganglia in the SAP group was significantly higher than that in the sham operated group (37.5 ± 12.28% vs 26.32 ± 16.15%, P <0.01). nNOS mRNA expression in ileum of SAP group was significantly higher than that in the sham operated group (1.02 ± 0.10 vs 0.70 ± 0.06, P < 0.01). The increased quantity of NOS-IR neurons in ileal myenteric ganglia and increased nNOS mRNA expression may suggest nNOS over expression as one of the nervous mechanisms of gastrointestinal dysmotility in SAP rat.