Characterization of three different types of extracellular vesicles and their impact on bacterial growth.
Characterization of three different types of extracellular vesicles and their impact on bacterial growth.
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DOI：
10.1016/j.foodchem.2018.08.059
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发表时间：
2019-01
期刊：
影响因子：
8.8
通讯作者：
Siran Yu;Zhehao Zhao;Xiaoyang Xu;Min Li;Ping Li
中科院分区：
文献类型：
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作者：
Siran Yu;Zhehao Zhao;Xiaoyang Xu;Min Li;Ping Li
最近发现体液中含有一类从细胞中释放出来的纳米颗粒，称为胞外囊泡；Exosome是一种小直径的胞外囊泡。我们选取了三种类型的样品：牛奶来源的外切体、脂肪来源的干细胞外切体和从椰子水中提取的纳米颗粒，研究它们的形态、粒度分布、蛋白质含量和microRNA的表达水平。在所研究的囊泡中，椰子纳米颗粒的大小分布最大，并且椰子纳米颗粒的蛋白质含量与哺乳动物外切体的蛋白质含量不同。利用荧光显微镜，我们确定了DiI标记的胞外囊泡可以被细菌吸收。乳源外切体能显著促进大肠杆菌MG1655和植物乳杆菌WCFS1的生长。所研究的胞外小泡可以改变细菌的基因表达。总体而言，本研究确定了不同来源的外源胞外小泡的差异，并揭示了它们对微生物生长的支持作用，以更好地利用微生物资源。
Recently body fluids have been found to contain a class of nanoparticles released from cells, referred to as extracellular vesicles; exosomes are a type of small-diameter extracellular vesicle. We selected three types of sample: milk-derived exosomes, adipose-derived stem cell exosomes, and nanoparticles extracted from coconut water, to investigate their morphology, particle size distribution, protein content, and microRNA expression levels. Among the vesicles investigated, coconut nanoparticles had the greatest size distribution, and the protein content of coconut nanoparticles differed from that of mammalian exosomes. Using fluorescence microscopy, we determined that DiI-labeled extracellular vesicles could be absorbed by bacteria. Prominently, milk-derived exosomes could promote the growth ofEscherichia coliK-12 MG1655 andLactobacillus plantarumWCFS1. The studied extracellular vesicles could alter bacterial gene expression. Overall, this study identified differences in exogenous extracellular vesicles from different sources and revealed their supportive effects on microbial growth to make better utilization of microbial resources.