Meis transcription factor maintains the neurogenic ectoderm and regulates the anterior-posterior patterning in embryos of a sea urchin, Hemicentrotus pulcherrimus
Meis transcription factor maintains the neurogenic ectoderm and regulates the anterior-posterior patterning in embryos of a sea urchin, Hemicentrotus pulcherrimus
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Meis 转录因子维持神经源性外胚层并调节海胆 Hemicentrotus pulcherrimus 胚胎的前后模式
DOI：
10.1016/j.ydbio.2018.09.018
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发表时间：
2018
影响因子：
2.7
通讯作者：
Yaguchi Shunsuke
中科院分区：
文献类型：
--
作者：
Yaguchi Junko;Yamazaki Atsuko;Yaguchi Shunsuke
精确的体轴形成是多细胞生物体发育的重要步骤，对于大多数多细胞生物体而言，卵中细胞命运决定因子的分子梯度和/或特异性偏向定位起着重要作用。然而，在海胆中，除了植物皮质分子外，尚未在卵中发现任何有偏见的蛋白质和mRNA，这表明海胆的发育主要受到均匀分布的母体分子的调节，这些分子对轴形成的贡献尚未得到很好的表征。在这里，我们描述了母亲Meis转录因子调节前后轴的形成，通过维护最前面的领土在胚胎的海胆，Hemicentrotus pulcherrimus。丧失功能的实验表明，Meis是本质上需要的前神经外胚层specifierfoxQ 2孵化后的维护，因此，morphant失去前神经外胚层的特点。此外，表达模式ofunivinandVEGF，外侧外胚层标记物，和间充质细胞模式向前方转移Meis morphants比他们在对照胚胎，表明Meis有助于精确的前后图案通过调节前神经外胚层的命运。
Precise body axis formation is an essential step in the development of multicellular organisms, for most of which the molecular gradient and/or specifically biased localization of cell-fate determinants in eggs play important roles. In sea urchins, however, any biased proteins and mRNAs have not yet been identified in the egg except for vegetal cortex molecules, suggesting that sea urchin development is mostly regulated by uniformly distributed maternal molecules with contributions to axis formation that are not well characterized. Here, we describe that the maternal Meis transcription factor regulates anterior-posterior axis formation through maintenance of the most anterior territory in embryos of a sea urchin,Hemicentrotus pulcherrimus. Loss-of-function experiments revealed that Meis is intrinsically required for maintenance of the anterior neuroectoderm specifierfoxQ2after hatching and, consequently, the morphant lost anterior neuroectoderm characteristics. In addition, the expression patterns ofunivinandVEGF, the lateral ectoderm markers, and the mesenchyme-cell pattern shifted toward the anterior side in Meis morphants more than they did in control embryos, indicating that Meis contributes to the precise anteroposterior patterning by regulating the anterior neuroectodermal fate.