Controlling ion channel function with renewable recombinant antibodies.
Controlling ion channel function with renewable recombinant antibodies.
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DOI：
10.1113/jp282403
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发表时间：
2022-05
影响因子：
5.5
通讯作者：
Trimmer, James S.
中科院分区：
文献类型：
--
作者：
Colecraft, Henry M.;Trimmer, James S.
选择性离子通道调节剂在生理学中起关键作用，其定义了特定离子通道对生理功能的贡献，并作为新型治疗策略的概念证明。抗体是有价值的研究工具，具有广泛的用途，包括定义天然组织中离子通道的表达和定位，以及捕获离子通道蛋白用于后续分析。在这篇综述中，我们详细介绍了如何可再生和重组抗体可用于控制离子通道功能。我们描述了不同形式的可再生和重组抗体，已被使用和它们调节离子通道功能的机制。我们强调使用细胞内表达的重组抗体（胞内抗体）作为遗传编码的工具来控制离子通道功能。我们还提供了未来研究的途径，可能会打开的新兴技术的应用工程重组抗体在离子通道研究中的效用增强。总体而言，这篇评论提供的见解，可能有助于刺激和指导感兴趣的研究人员开发和纳入可再生和重组抗体作为有价值的工具来控制离子通道功能。使用可再生重组抗体控制离子通道功能的两种不同方法。在左侧，外部应用的完整IgG抗体（紫色）与离子通道的细胞外结构域（浅蓝色）结合，以控制离子通道功能。在右侧，基因编码的胞内抗体，在该实施例中是与效应分子（红色）融合的骆驼纳米抗体（绿色），与离子通道的细胞内辅助亚基（深蓝色）结合以控制离子通道功能。
Selective ion channel modulators play a critical role in physiology in defining the contribution of specific ion channels to physiological function and as proof of concept for novel therapeutic strategies. Antibodies are valuable research tools that have broad uses including defining the expression and localization of ion channels in native tissue, and capturing ion channel proteins for subsequent analyses. In this review, we detail how renewable and recombinant antibodies can be used to control ion channel function. We describe the different forms of renewable and recombinant antibodies that have been used and the mechanisms by which they modulate ion channel function. We highlight the use of recombinant antibodies that are expressed intracellularly (intrabodies) as genetically-encoded tools to control ion channel function. We also offer perspectives of avenues of future research that may be opened by the application of emerging technologies for engineering recombinant antibodies for enhanced utility in ion channel research. Overall, this review provides insights that may help stimulate and guide interested researchers to develop and incorporate renewable and recombinant antibodies as valuable tools to control ion channel function. Two different approaches for controlling ion channel function using renewable recombinant antibodies. On the left, an externally applied intact IgG antibody (purple) binds to an extracellular domain of an ion channel (light blue) to control ion channel function. On the right, a genetically-encoded intrabody, in this example a camelid nanobody (green) fused to an effector molecule (red) binds to an intracellular auxiliary subunit of an ion channel (dark blue) to control ion channel function.
DOI：
10.1085/jgp.201311023
发表时间：
2013-09
期刊：
The Journal of general physiology
影响因子：
--
作者：
Sack JT;Stephanopoulos N;Austin DC;Francis MB;Trimmer JS
通讯作者：
Trimmer JS