Human epithelial cell death caused by Actinobacillus actinomycetemcomitans infection.
Human epithelial cell death caused by Actinobacillus actinomycetemcomitans infection.
复制标题
伴放线放线杆菌感染引起的人类上皮细胞死亡。
DOI：
10.1099/0022-1317-49-8-739
复制
发表时间：
2000
影响因子：
3
通讯作者：
Y. Kowashi
中科院分区：
文献类型：
--
作者：
S. Kato;K. Nakashima;M. Inoue;J. Tomioka;K. Nonaka;T. Nishihara;Y. Kowashi
牙龈沟是牙齿周围的浅裂隙，其上皮是牙周病初始细菌感染的门户。最近的研究表明，伴放线放线杆菌在体外侵入上皮细胞系KB细胞。本研究的目的是阐明A.伴放线菌感染A. MTT法测定感染后72、96和120 h KB细胞上放线菌共生菌的浓度。Hoechst 33258染色观察细胞核形态学变化。用ELISA法检测感染KB细胞的细胞质组蛋白相关DNA片段。A.伴放线菌对KB细胞具有细胞毒作用，细胞核浓缩、降解。感染后DNA断裂增加。此外，A.放线菌共生菌对人牙龈上皮细胞有类似的细胞毒作用。本研究表明，A.伴放线菌在体外培养系统中诱导口腔上皮细胞的凋亡性细胞死亡。这种诱导的细胞凋亡可能参与了牙周炎的发生和发展。
The gingival sulcus is the shallow crevice around the tooth, and its epithelium is a gateway for initial bacterial infection in periodontal disease. Recent studies have shown that Actinobacillus actinomycetemcomitans invades an epithelial cell line, KB cells, in vitro. The aim of the present study was to clarify the changes in KB cells after A. actinomycetemcomitans infection. The cytotoxic effects of A. actinomycetemcomitans on KB cells were determined at 72, 96 and 120 h after infection by an MTT assay. Nuclear morphological changes were observed by staining with Hoechst 33258. Cytoplasmic histone-associated DNA fragmentation in the infected KB cells was determined by ELISA. A. actinomycetemcomitans was cytotoxic on KB cells, and condensation and degradation of the nuclei were observed. DNA fragmentation was increased after the infection. In addition, A. actinomycetemcomitans showed similar cytotoxic effects on human gingival epithelial cells. The present study demonstrated that A. actinomycetemcomitans induces apoptotic cell death of oral epithelial cells in an in-vitro culture system. This induced apoptosis might be involved in the initiation and progression of periodontitis.