Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton
Application of liposome and stable isotope tracer techniques to study polyunsaturated fatty acid biosynthesis in marine zooplankton
复制标题
DOI：
10.1093/plankt/fbm025
复制
发表时间：
2007-05-01
影响因子：
2.1
通讯作者：
Pond, David W.
中科院分区：
文献类型：
--
作者：
Bell, Michael V.;Dick, James R.;Pond, David W.
采用脉冲追踪示踪法研究了4种海洋动物合成多不饱和脂肪酸（PUFAs）的能力。以脂质体D-5- 183 n-3为前体脂肪酸，对南极磷虾（Euphausiassuperba）的四种幼体：尖哲水蚤（Calanusfinmarchicus）、尖哲水蚤（Calanoidesacutus）、钳形哲水蚤（Drepanopusforcipatus）和大眼水蚤（Cylptopus）进行了室内饲养实验。虽然所有种类的浮游动物都容易摄取脂质体并将D-5-18：3 n-3示踪剂掺入其体细胞脂质池中，但只有可忽略的伸长和去饱和产物; D-5-18：4 n. D-5-20：5n-3和D-5-22：6n（-3）。我们的结论是，这里研究的四种海洋浮游动物无法合成PUPA在生态上显着的速度，当然不足以支持生长和繁殖过程的量。
We investigated the ability of four species of marine zooplanklon to synthesize polyunsaturated fato acids (PUFAs) during pulse-chase tracer experiments. Liposomes containing a deuterium labelled precursor fatty acid, D-5-183n-3, were fed to female Calanus finmarchicus, Calanoides acutus, Drepanopus forcipatus and caylptopus larvae of Euphausia superba during ship-board experiments. Although all species of zooplankton readily ingested the liposomes and incorporated the D-5-18:3n-3 tracer into their somatic lipid pool, only negligible products of elongation and desaturation; D-5-18:4n.-3, D-5-20:5n-3 and D-5-22:6n(-3) were detected after 96 h incubations. We conclude that the four species of marine zooplankton examined here are unable to synthesize PUPA at ecologically significant rates and certainly not in amounts sufficient to support growth and reproductive processes.