Evaluation of Basophil CD203c as a Predictor of Carboplatin-Related Hypersensitivity Reaction in Patients with Gynecologic Cancer
Evaluation of Basophil CD203c as a Predictor of Carboplatin-Related Hypersensitivity Reaction in Patients with Gynecologic Cancer
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DOI：
10.1248/bpb.b12-00150
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发表时间：
2012-09-01
影响因子：
2
通讯作者：
Okuda, Masahiro
中科院分区：
文献类型：
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作者：
Iwamoto, Takuya;Yuta, Atsushi;Okuda, Masahiro
据报道，重复使用卡铂后，卡铂过敏反应（HR）的发生率增加。然而，目前还没有可靠的体外试验来预测卡铂对HR的影响。在这项2009年10月至2010年9月在Mie大学医院进行的前瞻性病例对照研究中，我们评估了测量嗜碱性粒细胞CD203c预测卡铂相关HR的临床有效性。11例患者在过去3年内有卡铂相关HR病史，19例患者在接受卡铂治疗5个疗程以上后无HR病史。19例患者中有6例在研究期间发生卡铂相关HR。流式细胞仪分析CD203c+嗜碱性粒细胞（%）和平均荧光强度（MFI），并比较有无HR患者。在研究期间发生HR的患者中，还评估了HR前后嗜碱性细胞CD203c表达的变化。暴露于50 μ g/mL卡铂30分钟后，HR患者（3.5%,Delta MFI 9.0）的中位CD203c+嗜碱性粒细胞（%）和Delta MFI显著高于无HR患者（2.2%,Delta MFI 0.4）
The incidence of hypersensitivity reaction (HR) to carboplatin has been reported to increase after repeated use of the drug. However, a reliable ex vivo test to predict HR to carboplatin is not currently available. We evaluated the clinical usefulness of measuring basophil CD203c to predict carboplatin-related HR in this prospective case-control study conducted at Mie University Hospital between October 2009 and September 2010. Eleven patients had history of carboplatin-related HR within the past 3 years, and 19 had no history of HR after receiving more than 5 courses of carboplatin therapy. Six of these 19 patients developed carboplatin-related HR during the study period. The CD203c+ basophils (%) and the mean fluorescence intensity (MFI) were analyzed on a flow cytometer and compared between patients with and without HR. Changes in the CD203c expression on basophils before and after HR were also assessed in patients who developed HR during the study period. The median CD203c+ basophils (%) and Delta MFI after 30-min exposure to 50 mu g/mL carboplatin were significantly higher in patients with HR (3.5% and Delta MFI 9.0) compared with those without (2.2% and Delta MFI 0.4) (p