A basic helix-loop-helix protein with similarity to the fungal morphological regulators, Phd1p, Efg1p and StuA, controls conidiation but not dimorphic growth in Penicillium marneffei
A basic helix-loop-helix protein with similarity to the fungal morphological regulators, Phd1p, Efg1p and StuA, controls conidiation but not dimorphic growth in Penicillium marneffei
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DOI：
10.1046/j.1365-2958.2002.02906.x
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发表时间：
2002-05-01
影响因子：
3.6
通讯作者：
Andrianopoulos, A
中科院分区：
文献类型：
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作者：
Borneman, AR;Hynes, MJ;Andrianopoulos, A
APES蛋白家族的成员是碱性螺旋-环-螺旋(BHLH)蛋白，它调节真菌的交配、无性孢子形成和二态生长等过程。马尔尼菲青霉菌是一种人类病原体，是其属中唯一表现出二态生长转变的成员。在25℃时，马尔尼菲假单胞菌以丝状形态生长，并从多细胞分生孢子中产生无性孢子。在37℃时，丝状形态被通过分裂繁殖的酵母细胞所取代。我们克隆并鉴定了马尔尼菲青霉的APSES蛋白编码基因，该基因与尼杜拉曲霉有很高的相似性。Marneffei中StuA的缺失表明，它是分生孢子形成Metula和phialide所必需的，而不是二态生长所必需的。这表明APSES蛋白可能控制需要萌发的过程(变形层和菌丝的形成，酿酒酵母的假菌丝生长和白色念珠菌的二态生长)，但不控制那些需要分裂的过程(马尔尼菲假丝酵母的二态生长)。该突变株在分生孢子和交配过程中均存在缺陷。P，marneffei StuA基因能够补充突变株的分生孢子缺陷，但只能低效地补充突变株的性缺陷。这表明，来自无性物种的马尔尼菲P.marneffei基因在有性发育方面与有性发育基因存在显著差异，而不是无性发育。
Members of the APSES protein group are basic helix-loop-helix (bHLH) proteins that regulate processes such as mating, asexual sporulation and dimorphic growth in fungi. Penicillium marneffei is a human pathogen and is the only member of its genus to display a dimorphic growth transition. At 25 C, P. marneffei grows with a filamentous morphology and produces asexual spores from multicellular conidiophores. At 37 C, the filamentous morphology is replaced by yeast cells that reproduce by fission. We have cloned and characterized an APSES protein-encoding gene from P. marneffei that has a high degree of similarity to Aspergillus nidulans stuA. Deletion of stuA in P. marneffei showed that it is required for metula and phialide formation during conidiation but is not required for dimorphic growth. This suggests that APSES proteins may control processes that require budding (formation of the metulae and phialides, pseudohyphal growth in Saccharomyces cerevisiae and dimorphic growth in Candida albicans) but not those that require fission (dimorphic growth in P. marneffei). The A. nidulans DeltastuA mutant has defects in both conidiation and mating. The P, marneffei stuA gene was capable of complementing the conidiation defect but could only inefficiently complement the sexual defects of the A. nidulans mutant. This suggests that the P. marneffei gene, which comes from an asexual species, has diverged significantly from the A. nidulans gene with respect to sexual but not asexual development.