Blood Genomics in human stroke
Blood Genomics in human stroke
复制标题
DOI：
10.1161/01.str.0000250431.99687.7b
复制
发表时间：
2007-02-01
期刊：
影响因子：
8.3
通讯作者：
Baird, Alison E.
中科院分区：
文献类型：
--
作者：
Baird, Alison E.
微阵列技术和人类基因组测序的进展为基因组信息在临床医学中的应用开辟了新的可能性，利用有关DNA结构多态性和mRNA测量的基因表达变化的信息。癌症样本中的基因表达谱研究已经导致了临床特征的鉴定，这些临床特征已经在一些中心应用。在中风中，可以使用外周血作为mRNA的来源来研究基因表达。中风后，存在白色血细胞的选择性募集和迁移到脑中的缺血病灶。这似乎涉及所有白色细胞类型，并且据信一方面通过缺血性损伤的恶化，特别是在再灌注后，显著影响组织和临床结果，并且相反地有助于中风后数天至数周的组织重塑和修复。缺血性中风临床研究的第一个结果表明，基因表达特征可以从外周白色血细胞中得到证实，这至少代表了对改变的大脑微环境的部分适应。进一步的研究表明，这些方法是否可能导致新的管理方法中风。（中风。2007;38[第2部分]：694-698。
Advances in microarray technology and the sequencing of the human genome are opening up new possibilities for applying genomic information in clinical medicine, using information about structural polymorphisms in DNA and changes in gene expression as measured by mRNA. Gene expression profiling studies in cancer samples have led to the identification of clinical signatures that are already being applied in some centers. In stroke, it may be possible to use peripheral blood as a source of mRNA to study gene expression. After stroke, there is a selective recruitment and migration of white blood cells to the ischemic focus in the brain. This appears to involve all white cell types and is believed to impact significantly on tissue and clinical outcome through the exacerbation of ischemic injury, particularly after reperfusion, on the one hand, and conversely contributing to tissue remodeling and repair days to weeks after stroke. The first results from clinical studies in ischemic stroke suggest that a gene expression signature can be demonstrated from peripheral white blood cells and that this represents at least a partial adaptation to the altered cerebral microenvironment. Further studies are indicated to see whether these methods may lead to new management approaches for stroke. (Stroke. 2007;38[part 2]: 694-698.)