Serum HLA class I antigens: markers and modulators of an immune response?
Serum HLA class I antigens: markers and modulators of an immune response?
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DOI：
10.1016/0167-5699(95)80127-8
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发表时间：
1995-03
期刊：
影响因子：
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通讯作者：
F. Puppo;M. Scudeletti;F. Indiveri;S. Ferrone
中科院分区：
文献类型：
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作者：
F. Puppo;M. Scudeletti;F. Indiveri;S. Ferrone
20多年前，主要组织相容性复合体(MHC)的HLA-A2和-B7抗原被报道存在于血清中。2.当时认为，血清中的HLAI类抗原可能是由肝细胞产生的，它们可能通过诱导对不匹配的HLAI类同种异体特异性的无反应性而对同种异体移植物的存活产生有利的影响。在接下来的几年里，研究的重点是评估血清作为人类白细胞抗原配型、结构研究和发展抗人类白细胞抗原异种抗体炎的抗原来源。通过抑制补体依赖的细胞毒作用，所有已知的人类白细胞抗原I类同种异体特异性都在血清中表达，尽管水平不同。然而，它很快就变得明显，这种方法不是一种实用的方法来进行人类白细胞抗原I类表型。此外，尽管从血清中提纯的人类白细胞抗原I类抗原的早期研究提供了关于其分子特征的有用信息，但细胞培养技术的进步有利于使用B细胞系作为人类白细胞抗原的来源来进行结构表征。在兔和山羊体内，血清中的HLAI类抗原比从淋巴样细胞中提纯的抗原更能有效地诱导出具有手术特异性的抗HLAI类抗血清。然而，由于对异种抗血清用于人类白细胞抗原分型的有效性持怀疑态度，这一研究没有发展起来。此外，血清HLAHLA1类抗原缺乏免疫原性，以可溶性形式在血清中循环。本文讨论了病毒性疾病患者和器官移植术后患者血清HL4-I类抗原水平的临床意义，以及此类抗原参与免疫反应的可能性。
More than 20 years ago, the major histocompatibility complex (MHC) HLA-A2 and-B7 antigens were reported to be present in serum’. 2. At that time, it was suggested that serum HLA class I antigens may be produced by hepatocytes, and that they may have a beneficial effect on allograft survival by inducing unresponsiveness to mismatched HLA class I allospecificities. In the following years, investigations focused on the evaluation of serum as a source of antigens for HLA typing, for structural studies and for development of anti-HLA xenoantiboditis. By utilizing inhibition of complement-dependent cytotoxicity, all known HLA class I allospecificities were found to be expressed in serum, although at different levels. Nevertheless, it quickly became apparent that this method was not a practical approach for HLA class I phenotyping. Furthermore, although early studies with HLA class I antigens purified from serum had provided useful information concerning their molecular profile, progress in cell culture technology favoured the use of B-cell lines as a source of HLA antigens for structural characterization. Lastly, serum HLA class I antigens were found to be markedly more efficient than antigens purified from lymphoid cells in eliciting operationally specific anti-HLA class I antisera in rabbits and goats. However, this line of research was not developed because of scepticism concerning the usefulness of xenoantisera for HLA typing. Furthermore, the lack of immunogenicity of serum HLAHLA class 1 antigens circulate in the serum in soluble form. This article discusses the clinical significance of levels of serum HL4 class I antigens, both in patients with viral diseases and following organ transplantation, as well as the potential involvement of such antigens in the immune response.