KIFC2 is a novel neuron-specific C-terminal type kinesin superfamily motor for dendritic transport of multivesicular body-like organelles
KIFC2 is a novel neuron-specific C-terminal type kinesin superfamily motor for dendritic transport of multivesicular body-like organelles
复制标题
DOI：
10.1016/s0896-6273(00)81243-x
复制
发表时间：
1997-03-01
期刊：
影响因子：
16.2
通讯作者：
Hirokawa, N
中科院分区：
文献类型：
--
作者：
Saito, N;Okada, Y;Hirokawa, N
我们从小鼠脑中克隆了两个新的C-末端马达结构域型驱动蛋白超家族马达蛋白（KIFC）。第一种蛋白质是CHO 2抗原的鼠同源物，是kar 3型有丝分裂马达亚家族的成员，我们将这种蛋白质命名为KIFC 1。另一种蛋白KIFC 2（792个氨基酸）是新的，与已知的驱动蛋白超家族蛋白（KIF）没有显着相似性。KIFC 2在成年神经元中特异性表达，并且免疫荧光定位于细胞体和树突中的点状结构，但在轴突中未检测到。免疫分离的KIFC 2结合细胞器的电子显微镜分析显示，KIFC 2与多泡体（mvb）样细胞器，这表明KIFC 2的功能作为电机的mvb样细胞器在树突中的运输。
We have cloned two novel C-terminal motor domain-type kinesin superfamily motor proteins (KIFCs) from mouse brain by utilizing a KIFC-specific consensus sequence. The first protein was the murine homologue of CHO2 antigen, a member of the kar3-type mitotic motor subfamily, and we designated this protein KIFC1. The other protein, KIFC2 (792 amino acids), is novel, with no significant similarity to known kinesin superfamily proteins (KIFs). KIFC2 was specifically expressed in adult neurons, and was immunofluorescently localized to punctate structures in cell bodies and dendrites, but was not detected in axons. Electron microscopic analysis of the immunoisolated KIFC2-bound organelles revealed that KIFC2 associates with multivesicular body (mvb)-like organelles, suggesting that KIFC2 functions as the motor for the transport of mvb-like organelles in dendrites.