Identification of odorant-binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana
Identification of odorant-binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana
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DOI：
10.1111/imb.12328
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发表时间：
2017-12-01
影响因子：
2.6
通讯作者：
He, M.
中科院分区：
文献类型：
--
作者：
He, P.;Li, Z. -Q.;He, M.
美洲蟑螂（Periplaneta americana）是一种城市害虫，具有精确的化学感受系统，可帮助其实现复杂的生理行为，包括定位食物和交配。然而，其化学感受机制尚未得到很好的研究。在这里，我们确定了71个假定的气味载体蛋白基因在美洲大蠊，包括57个新的气味结合蛋白（OBP）和11个化学感受蛋白（CSP）。为了确定它们的生理功能，我们研究了它们在触角、口器、腿和两性身体其余部分的组织表达模式，并确定这些基因中的大多数在化学感受器官中表达。系统进化树显示美洲大蠊与蛾类信息素结合蛋白属于不同的进化分支。两个基因，PameOBP24和PameCSP7，同样表达在两种性别的触角和高表达之间的OBPs和CSP。这些基因在大肠杆菌中表达，并纯化所得蛋白。用重组PameOBP24和PameCSP7测试了74种常见气味化合物的结合亲和力。这两种蛋白质结合了多种配体。我们的研究结果为未来研究美洲大蠊的化学感受机制提供了基础，并有助于确定管理这种害虫的潜在靶基因。
The American cockroach (Periplaneta americana) is an urban pest with a precise chemosensory system that helps it achieve complex physiological behaviours, including locating food and mating. However, its chemosensory mechanisms have not been well studied. Here, we identified 71 putative odorant carrier protein genes in P.americana, including 57 new odorant-binding proteins (OBPs) and 11 chemosensory proteins (CSPs). To identify their physiological functions, we investigated their tissue expression patterns in antennae, mouthparts, legs, and the remainder of the body of both sexes, and determined that most of these genes were expressed in chemosensory organs. A phylogenetic tree showed that the putative pheromone-binding proteins of P.americana were in different clades from those of moths. Two genes, PameOBP24 and PameCSP7, were expressed equally in antennae of both sexes and highly expressed amongst the OBPs and CSPs. These genes were expressed in Escherichia coli and the resultant proteins were purified. The binding affinities of 74 common odorant compounds were tested with recombinant PameOBP24 and PameCSP7. Both proteins bound a variety of ligands. Our findings provide a foundation for future research into the chemosensory mechanisms of P.americana and help in identifying potential target genes for managing this pest.