18-P015 3D analysis of gene expression during limb development in the Chick
18-P015 3D analysis of gene expression during limb development in the Chick
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18-P015 鸡肢体发育过程中基因表达的 3D 分析
DOI：
10.1016/j.mod.2009.06.782
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发表时间：
2009
影响因子：
2.6
通讯作者：
Welten M
中科院分区：
文献类型：
--
作者：
Welten M
在肢芽后缘的极化区域中的Shh信号传导在控制发育中的肢的数字和模式中是关键的。使用微阵列，我们比较了野生型和talpid [3]突变型鸡胚翅芽前三分之一和后三分之一的基因表达水平，其中Gli激活子和阻遏子功能均失败，并确定了1070个差异表达基因。这些基因被分成16个簇，其中一个包含Hoxd13，一种已知参与手指形成的基因。使用光学投影断层扫描（OPT），我们已经进行了3D分析的Hoxd13样簇中的基因的表达模式。通过这种分析，我们的目标是确定新的基因，作为Shh信号转导的结果，因此在鸡翅发育中发挥作用。然后使用AMIRA软件将3D基因表达模式映射到数字参考肢体上，并使用计算分析来比较基因表达的3D空间模式，这可能会识别提示肢体发育中潜在功能关系的同基因表达组。
Shh signalling in the polarizing region at the posterior edge of the limb bud is pivotal in controlling digit number and pattern in the developing limb. Using microarrays we have compared gene expression levels between anterior and posterior thirds of wing buds from wild-type and talpid [3] mutant chicken embryos, in which both Gli activator and repressor function fail, and identified 1070 differentially expressed genes. These were put into 16 clusters, one of which contains Hoxd13, a gene known to be involved in digit formation. Using optical projection tomography (OPT) we have performed a 3D analysis of the expression patterns of genes in the Hoxd13-like cluster. Through this analysis we aim to identify novel genes that are modulated as a consequence of Shh signaling and therefore play a role in chicken wing development. The 3D gene expression patterns were then mapped onto a digital reference limb using AMIRA software and computational analysis was used to compare the 3D spatial patterns of gene expression, which may possibly identify syn-expression groups suggesting potential functional relationships in limb development.