Stimulated endocytosis in penetratin uptake:: Effect of arginine and lysine
Stimulated endocytosis in penetratin uptake:: Effect of arginine and lysine
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DOI：
10.1016/j.bbrc.2008.04.039
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发表时间：
2008-07-11
影响因子：
3.1
通讯作者：
Esbjorner, Elin K.
中科院分区：
文献类型：
--
作者：
Amand, Helene L.;Fant, Kristina;Esbjorner, Elin K.
细胞穿透肽可以将大分子货物递送到细胞中，并显示出作为细胞内药物递送载体的前景。内化主要通过内吞作用发生，但确切的摄取机制尚不完全清楚。我们定量地显示如何penetratin，16个残基的阳离子肽，刺激液相内吞作用，并触发其自身的摄取到中国仓鼠卵巢细胞，使用70 kDa的葡聚糖，以表明巨胞饮。总的细胞内吞率的影响显着较小，因此，我们提出了上调巨胞饮发生在其他类型的内吞的代价。通过比较penetratin其类似物PenArg和PenLys，丰富的精氨酸和赖氨酸，分别，我们展示了这些侧链如何有助于吸收效率。肽和葡聚糖摄取的程度遵循关于肽浓度和精氨酸/赖氨酸含量的类似模式（PenArg >穿透素> PenLys），表明高含量的精氨酸对于刺激内吞作用是有益的但不是必需的。(C)2008年爱思唯尔公司All rights reserved.
Cell-penetrating peptides can deliver macromolecular cargo into cells and show promise as vectors for intracellular drug delivery. Internalization occurs predominantly via endocytosis, but the exact uptake mechanisms are not fully understood. We show quantitatively how penetratin, a 16-residue cationic peptide, stimulates fluid-phase endocytosis and triggers its own uptake into Chinese hamster ovarian cells, using a 70 kDa dextran to indicate macropinocytosis. The total cellular endocytotic rate is significantly less affected and we therefore propose up-regulation of macropinocytosis to occur at the expense of other types of endocytosis. By comparing penetratin to its analogs PenArg and PenLys, enriched in arginines and lysines, respectively, we show how these side-chains contribute to uptake efficiency. The degree of peptide and dextran uptake follows similar patterns regarding peptide concentration and arginine/lysine content (PenArg > penetratin > PenLys), indicating that a high content of arginines is beneficial but not necessary for stimulating endocytosis. (C) 2008 Elsevier Inc. All rights reserved.