Potentiation of serum response factor activity by a family of myocardin-related transcription factors
Potentiation of serum response factor activity by a family of myocardin-related transcription factors
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DOI：
10.1073/pnas.222561499
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发表时间：
2002-11-12
影响因子：
11.1
通讯作者：
Olson, EN
中科院分区：
文献类型：
--
作者：
Wang, DZ;Li, SJ;Olson, EN
肌心素是SAP（SAF-A/B，腺泡，皮亚斯）结构域转录因子，其与血清应答因子（SRF）结合以有效地增强SRF依赖性转录。在这里，我们描述了两种肌红蛋白相关转录因子（MRTF）A和B，它们也与SRF相互作用并刺激其转录活性。而myocardin特异性地表达于心脏和平滑肌细胞中，MRTF-A和-B在许多胚胎和成人组织中表达。在SRF缺陷的胚胎干细胞中，myocardin和MRTF不能激活SRF依赖的报告基因，证实了它们对SRF的依赖。肌心素和MRTF包含一个以前未表征的SRF辅因子家族，具有在广泛组织中调节SRF靶基因的潜力。
Myocardin is a SAP (SAF-A/B, Acinus, PIAS) domain transcription factor that associates with serum response factor (SRF) to potently enhance SRF-dependent transcription. Here we describe two myocardin-related transcription factors (MRTFs), A and B, that also interact with SRF and stimulate its transcriptional activity. Whereas myocardin is expressed specifically in cardiac and smooth muscle cells, MRTF-A and -B are expressed in numerous embryonic and adult tissues. In SRF-deficient embryonic stem cells, myocardin and MRTFs are unable to activate SRF-dependent reporter genes, confirming their dependence on SRF. Myocardin and MRTFs comprise a previously uncharacterized family of SRF cofactors with the potential to modulate SRF target genes in a wide range of tissues.