Primers for low-copy nuclear genes in the Melastomataceae.
Primers for low-copy nuclear genes in the Melastomataceae.
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DOI：
10.3732/apps.1500092
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发表时间：
2016-01
影响因子：
3.6
通讯作者：
Michelangeli FA
中科院分区：
文献类型：
--
作者：
Reginato M;Michelangeli FA
低拷贝的核基因引物开发的系统发育研究，在整个Mexalataceae。将来自八个种的全基因组文库连同一个转录组一起用于标记鉴定和引物设计，所述八个种在木贼科中沿着。8个外显子引物的内含子交叉标记扩增成功的9族的分类群中的Mexalataceae。新的标记直接测序的8个样品的密切相关的物种的Miconiae（Chaenanthera分支）。8个位点的DNA序列范围从660到818对齐的碱基对。与其他研究中常用的四种标记相比，这里开发的基因座具有比质体间隔区更多的可变位点（7-16对26-45）和与核糖体间隔区相当的变异（28-39）。新的引物对应该是有用的广泛的系统学和进化的研究，在不同的Mendriataceae。
Low-copy nuclear gene primers were developed for phylogenetic studies across the Melastomataceae. Total genomic libraries from eight species in the Melastomataceae along with one transcriptome were used for marker identification and primer design. Eight exon-primed intron-crossing markers were amplified with success in taxa of nine tribes in the Melastomataceae. The new markers were directly sequenced for eight samples of closely related species of Miconia (Chaenanthera clade) in the tribe Miconieae. The DNA sequences for the eight loci ranged from 660 to 818 aligned base pairs. Compared with four commonly used markers in other studies, the loci developed here had a higher number of variable sites than plastid spacers (7–16 vs. 26–45) and comparable variation to the ribosomal spacers (28–39). The novel primer pairs should be useful for a broad range of studies of systematics and evolution in the diverse Melastomataceae.