Biological oxidation of dissolved methane in effluents from anaerobic reactors using a down-flow hanging sponge reactor
Biological oxidation of dissolved methane in effluents from anaerobic reactors using a down-flow hanging sponge reactor
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DOI：
10.1016/j.watres.2009.11.021
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发表时间：
2010-03-01
期刊：
影响因子：
12.8
通讯作者：
Ohashi, Akiyoshi
中科院分区：
文献类型：
--
作者：
Hatamoto, Masashi;Yamamoto, Hiroki;Ohashi, Akiyoshi
厌氧废水处理厂排放溶解的甲烷，通常无法回收。为了防止温室气体甲烷的排放，我们使用了一个密封的下流悬挂式海绵反应器作为后处理，以生物氧化溶解的甲烷。在反应器启动后的3周内，在HRT为2 h的条件下，甲烷去除率达到95%，甲烷去除率为0.8 kg COD m（3）d（-1）。当HRT为0.5h时，提高甲烷负荷后，最大甲烷去除率可达2.2kgCOD·m ~（-3）·d ~（-1）。另一方面，在第一阶段，只有约10%的进水铵被氧化为硝酸盐，但当气流增加到2.5 L/天（-1）时，硝化效率增加到约70%。然而，氨的氧化速率则随着甲烷负荷率的增加而降低。这些结果表明，在反应器中，甲烷氧化优先于铵氧化发生。16 S rRNA和pmoA基因的克隆以及系统发育和T-RFLP分析表明，I型甲烷氧化菌是占主导地位的甲烷氧化菌，而II型甲烷氧化菌仅在反应器的一小部分检测。(C)2009爱思唯尔有限公司保留所有权利。
Anaerobic wastewater treatment plants discharge dissolved methane, which is usually not recovered. To prevent emission of methane, which is a greenhouse gas, we utilized an encapsulated down-flow hanging sponge reactor as a post-treatment to biologically oxidize dissolved methane. Within 3 weeks after reactor start-up, methane removal efficiency of up to 95% was achieved with a methane removal rate of 0.8 kg COD m(3) day(-1) at an HRT of 2 h. After increasing the methane-loading rate, the maximum methane removal rate reached 2.2 kg COD m(-3) day(-1) at an HRT of 0.5 h. On the other hand, only about 10% of influent ammonium was oxidized to nitrate during the first period, but as airflow was increased to 2.5 L day(-1), nitrification efficiency increased to approximately 70%. However, the ammonia oxidation rate then decreased with an increase in the methane-loading rate. These results indicate that methane oxidation occurred preferentially over ammonium oxidation in the reactor. Cloning of the 16S rRNA and pmoA genes as well as phylogenetic and T-RFLP analyses revealed that type I methanotrophs were the dominant methane oxidizers, whereas type II methanotrophs were detected only in minor portion of the reactor. (C) 2009 Elsevier Ltd. All rights reserved.