Expression Level of ABCG2 in the Placenta Decreases from the Mid Stage to the End of Gestation
Expression Level of ABCG2 in the Placenta Decreases from the Mid Stage to the End of Gestation
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DOI：
10.1271/bbb.69.1871
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发表时间：
2005-01
期刊：
影响因子：
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通讯作者：
S. Yasuda;S. Itagaki;T. Hirano;K. Iseki
中科院分区：
文献类型：
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作者：
S. Yasuda;S. Itagaki;T. Hirano;K. Iseki
本研究旨在阐明ABCG2在妊娠中期至晚期胎盘中的表达模式。分别于孕14天(Gd)和20 d观察rABCG2的表达。RT-PCR法检测胎盘组织中rABCG2基因的表达，Western印迹法检测胎盘组织中rABCG2蛋白的表达。胎盘分泌的孕酮对BeWo细胞中ABCG2的表达有调节作用。孕酮处理可降低BeWo细胞中ABCG2基因和蛋白的表达水平。结论：孕酮在胎盘ABCG2表达水平的降低中起一定作用，且随着孕周的延长，ABCG2的表达水平逐渐降低。
The aim of this study was to elucidate the expression pattern of ABCG2 in the placenta from the mid stage to the end of gestation. rABCG2 expression was investigated in rats on the 14th gestation day (gd) and the 20th gd. Expression of the rABCG2 gene and expression of rABCG2 protein in the placenta were detected on gd 14 by RT-PCR and Western blot analysis respectively. The expression level of rABCG2 on gd 20 was less than that on gd 14. We investigated whether progesterone, secreted from the placenta, regulates the expression of ABCG2 in BeWo cells. Expression levels of the ABCG2 gene and protein in BeWo cells were decreased by progesterone treatment. We conclude that progesterone plays a role in reduction in the expression level of ABCG2 in the placenta with the advance of gestation from the mid stage to the end of gestation.