Metabolic Profiling of Synovial Fluid in a Unilateral Ovine Model of Anterior Cruciate Ligament Reconstruction of the Knee Suggests Biomarkers for Early Osteoarthritis
Metabolic Profiling of Synovial Fluid in a Unilateral Ovine Model of Anterior Cruciate Ligament Reconstruction of the Knee Suggests Biomarkers for Early Osteoarthritis
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DOI：
10.1002/jor.22743
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发表时间：
2015-01-01
影响因子：
2.8
通讯作者：
Vogel, Hans J.
中科院分区：
文献类型：
--
作者：
Mickiewicz, Beata;Heard, Bryan J.;Vogel, Hans J.
关节损伤和随后的骨关节炎（OA）是慢性关节疾病的主要原因。在这项工作中，我们探索了应用基于磁共振波谱的代谢组学来检测宿主对绵羊模型滑液中前交叉韧带（ACL）重建损伤的反应的可能性。使用多变量统计分析，我们能够区分受伤后的关节样本（ACL 和假手术）与未受伤的对照样本，以及来自假手术的 ACL 手术样本。所有样本中均定量了 65 种代谢物，其中 6 种可作为膝关节损伤后早期退行性变化的生物标志物：异丁酸、葡萄糖、羟脯氨酸、天冬酰胺、丝氨酸和尿苷。我们的研究结果提出了一个警告，表明对膝盖进行手术干预可能会导致代谢改变，需要将其与早期发生的 OA 引起的代谢改变区分开来。我们的研究结果说明了代谢组学作为检测膝关节损伤的诊断和预后工具的潜在应用。检测绵羊滑液中独特的代谢变化模式的能力为未来将这种方法扩展到患者人群的精准医疗方案提供了可能性。 (c) 2014 年骨科研究学会。由 Wiley periodicals, Inc. 出版。J Orthop Res 33:71-77，2015 年。
Joint injuries and subsequent osteoarthritis (OA) are the leading causes of chronic joint disease. In this work, we explore the possibility of applying magnetic resonance spectroscopy-based metabolomics to detect host responses to an anterior cruciate ligament (ACL) reconstruction injury in synovial fluid in an ovine model. Using multivariate statistical analysis, we were able to distinguish post-injury joint samples (ACL and sham surgery) from the uninjured control samples, and as well the ACL surgical samples from sham surgery. In all samples there were 65 metabolites quantified, of which six could be suggested as biomarkers for early post-injury degenerative changes in the knee joints: isobutyrate, glucose, hydroxyproline, asparagine, serine, and uridine. Our results raise a cautionary note indicating that surgical interventions into the knee can result in metabolic alterations that need to be distinguished from those caused by the early onset of OA. Our findings illustrate the potential application of metabolomics as a diagnostic and prognostic tool for detection of injuries to the knee joint. The ability to detect a unique pattern of metabolic changes in the synovial fluid of sheep offers the possibility of extending the approach to precision medicine protocols in patient populations in the future. (c) 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:71-77, 2015.