Aggregates of acetylcholinesterase induced by acetylcholine receptor-aggregating factor.
Aggregates of acetylcholinesterase induced by acetylcholine receptor-aggregating factor.
复制标题
由乙酰胆碱受体聚集因子诱导的乙酰胆碱酯酶聚集。
作者：
Wallace,BG;Nitkin,RM;Reist,NE;Fallon,JR;Moayeri,NN;McMahan,UJ
加利福尼亚电器官的基底层丰富提取物含有一种因子，可使培养的肌管上的乙酰胆碱受体（achr）聚集成斑块1 - 3。我们之前的研究表明，这些提取物的活性成分类似于基底层的分子，这些分子在体内再生神经肌肉连接时指导肌纤维质膜中achr的聚集2,4,5。因为它可以大量获得并在细胞培养的受控条件下进行分析，所以来自电器官的achr聚集因子对于详细检查再生肌肉的突触后装置是如何组装的特别有用。在这里，我们证明了电器官因子不仅引起培养肌管上AChR聚集体的形成，而且引起乙酰胆碱酯酶（AChE）斑块的形成。这一发现，再加上观察到在体内再生的神经肌肉连接处，基底膜指导AChR和AChE聚集物的形成，使我们假设突触基底膜的单一成分导致再生肌纤维上这两种突触特化的形成。
Basal lamina-rich extracts ofTorpedo californicaelectric organ contain a factor that causes acetylcholine receptors (AChRs) on cultured myotubes to aggregate into patches1–3. Our previous studies have indicated that the active component of these extracts is similar to the molecules in the basal lamina which direct the aggregation of AChRs in the muscle fibre plasma membrane at regenerating neuromuscular junctionsin vivo2,4,5. Because it can be obtained in large amounts and assayed in controlled conditions in cell culture, the AChR-aggregating factor from electric organ may be especially useful for examining in detail how the postsynaptic apparatus of regenerating muscle is assembled. Here we demonstrate that the electric organ factor causes not only the formation of AChR aggregates on cultured myotubes, but also the formation of patches of acetylcholinesterase (AChE). This finding, together with the observation that basal lamina directs the formation of both AChR and AChE aggregates at regenerating neuromuscular junctionsin vivo6, leads us to hypothesize that a single component of the synaptic basal lamina causes the formation of both these synaptic specializations on regenerating myofibres.