Effects of electroacupuncture at Zusanli (ST36) on inflammatory cytokines in a rat model of smoke-induced chronic obstructive pulmonary disease
Effects of electroacupuncture at Zusanli (ST36) on inflammatory cytokines in a rat model of smoke-induced chronic obstructive pulmonary disease
复制标题
电针足三里对烟雾性慢性阻塞性肺疾病大鼠模型炎症细胞因子的影响
DOI：
10.3736/jintegrmed2013024
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发表时间：
2013-05-01
影响因子：
4.8
通讯作者：
Shen, Lin-lin
中科院分区：
文献类型：
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作者：
Geng, Wen-ye;Liu, Zi-bing;Shen, Lin-lin
目的：针灸治疗慢性阻塞性肺疾病（COPD）后肺功能有所改善，但其潜在机制尚不清楚。由于在COPD中可以看到免疫反应失衡，我们假设电针（EA）可能在调节炎症细胞因子中发挥作用，并有助于烟雾诱导的COPD大鼠模型的肺保护。方法：用暴露于香烟烟雾中的雄性Sprague-Dawley大鼠建立COPD模型。将大鼠随机分为4组（对照组、假手术组、COPD组、COPD + EA组），通过测量肺病理变化和肺功能评价COPD模型。假性慢性阻塞性肺病大鼠和COPD大鼠分别在足三里穴（ST36）应用EA穴位30 min/d，连续14 d。采用支气管肺泡灌洗液（BALF）测定肿瘤坏死因子- α (tnf - α)、白细胞介素-1 β (IL-1 β)和丙二醛（MDA）水平。结果：与对照组大鼠相比，COPD大鼠肺阻力（R-L）和肺顺应性（C-L）均发生显著变化
OBJECTIVE: Improvement in lung function was reported after acupuncture treatment of chronic obstructive pulmonary disease (COPD), but little is known about the underlying mechanisms. Because an immune response imbalance could be seen in COPD, we hypothesize that electroacupuncture (EA) may play a role in regulating inflammatory cytokines and contribute to lung protection in a rat model of smoke-induced COPD.METHODS: A COPD model using male Sprague-Dawley rats exposed to cigarette smoke was established. The rats were randomly divided into four groups (control, sham, COPD, and COPD plus EA), and COPD model was evaluated by measuring pulmonary pathological changes and lung function. EA was applied to the acupuncture point Zusanli (ST36) for 30 min/d for 14 d in sham and COPD rats. Bronchoalveolar lavage fluid (BALF) was used to measure levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and malonaldehyde (MDA).RESULTS: Compared with the control rats, COPD rats had significant changes in lung resistance (R-L) and lung compliance (C-L) (both P