Retention of endothelial cell adherence to porcine-derived extracellular matrix after disinfection and sterilization
Retention of endothelial cell adherence to porcine-derived extracellular matrix after disinfection and sterilization
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DOI：
10.1089/107632702753724996
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发表时间：
2002-04-01
影响因子：
--
通讯作者：
Badylak, SF
中科院分区：
文献类型：
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作者：
Hodde, JP;Record, RD;Badylak, SF
来源于猪组织的细胞外基质（ECM）在用作体内组织修复的支架时与宿主细胞的快速和广泛的再增殖相关。细胞粘附到用于组织工程的基底上已经被广泛研究，但是在用氧化剂和灭菌剂处理后的ECM支架中介导这种现象的因素还没有被检查。细胞粘附试验用于检查人微血管内皮细胞（HMEC）附着ECM移植材料收获小肠粘膜下层（SIS）和膀胱基质（UBM）的脱细胞和灭菌程序，旨在使ECM安全用于临床使用。HMEC能够通过ECM内存在的几种附着蛋白直接附着到这些ECM支架上，包括I型胶原、IV型胶原和纤连蛋白。还检查了SIS ECM和UBM ECM支持HMEC生长和增殖的能力。HMEC能够在SIS和UBM片材的两个表面上生长至单层汇合。如果将内皮细胞接种在ECM片的近腔侧，则它们也能够在稍后的时间点穿透SIS和UBM。处理过的ECM支持HMEC附着和增殖的能力与未处理ECM的报道相似，因此可能在这些基质作为伤口修复支架植入体内时观察到的快速重塑反应中发挥作用。
Extracellular matrices (ECM) derived from porcine tissue are associated with rapid and extensive repopulation with host cells when used as scaffolds for in vivo tissue repair. Cell adhesion to substrates used for tissue engineering has been studied extensively but the factors that mediate this phenomenon in ECM scaffolds following treatment with oxidants and sterilants have not been examined. Cell adhesion assays were used to examine human microvascular endothelial cell (HMEC) attachment to ECM graft materials harvested from small intestinal submucosa (SIS) and urinary bladder matrix (UBM) following decellularization and sterilization procedures designed to render the ECM safe for clinical use. HMECs were able to attach directly to these ECM scaffolds via several attachment proteins present within the ECM, including type I collagen, type IV collagen, and fibronectin. The ability of the SIS ECM and UBM ECM to support the growth and proliferation of HMEC was also examined. HMEC were able to grow to single-layer confluence on both surfaces of SIS and UBM sheets. The endothelial cells were also able to penetrate the SIS and UBM at later time points if they were seeded on the abluminal side of the ECM sheets. The ability of the processed ECM to support HMEC attachment and proliferation is similar to that reported for unprocessed ECM and may therefore play a role in the rapid remodeling response observed when these matrices are implanted in vivo as scaffolds for wound repair.