A Scanning Frequency Mode for Ion Cyclotron Mobility Spectrometry
A Scanning Frequency Mode for Ion Cyclotron Mobility Spectrometry
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DOI：
10.1021/ac1017474
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发表时间：
2010-10-01
影响因子：
7.4
通讯作者：
Clemmer, David E.
中科院分区：
文献类型：
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作者：
Glaskin, Rebecca S.;Valentine, Stephen J.;Clemmer, David E.
探索了离子回旋迁移率谱仪的一种新的操作模式，作为进行高分辨率分离的可能手段。该方法基于应用于圆形漂移管的分段区域的振荡场。具有与电场应用频率共振的迁移率的离子被传输，而不共振的离子被消除。通过对漂移场应用频率的扫描，得到了离子迁移谱。该方法通过检测电喷涂P肽物质产生的离子混合物以及酶切细胞色素c获得的色氨酸肽混合物来证明。P肽物质离子的漂移场应用频率扫描表明，可以分离[M+2H]（2+）离子，以及紧凑和细长形式的[M+3H]（3+）离子。不同离子的分辨率与分析的循环次数有关。在高周数（bbb50 3/4或漂移长度为9242.03 cm）时，分辨率值可超过300，最大分辨率接近400。通过改变离子循环数来调整基于迁移率的分离的分辨能力的能力具有重要的分析效用。
A new operational mode for an ion cyclotron mobility spectrometry instrument is explored as a possible means of performing high-resolution separations. The approach is based on oscillating fields that are applied to segmented regions of a circular drift tube. Ions with mobilities that are resonant with the frequency of field application are transmitted while nonresonant species are eliminated. An ion mobility spectrum is obtained by scanning the drift field application frequency. The approach is demonstrated by examining mixtures of ions produced by electrospraying the substance P peptide, as well as a mixture of tryptic peptides obtained by enzymatic digestion of cytochrome c. Drift field application frequency scans of substance P peptide ions show that it is possible to separate [M+2H](2+) ions, and compact and elongated forms of [M+3H](3+) ions. The resolution of different ions is related to the number of cycles for the analysis. At high cycle numbers (>50 3/4 or a drift length of 9242.03 cm) values of the resolving power can exceed 300 with a maximum resolving power of similar to 400. The ability to tune the resolving power of a mobility-based separation by varying the ion cycle number has substantial analytical utility.