Nanofabricated carbon-based detector.
Nanofabricated carbon-based detector.
复制标题
纳米碳基探测器。
DOI：
10.1021/ac0487404
复制
发表时间：
2005
影响因子：
7.4
通讯作者：
Parpura,Vladimir
中科院分区：
文献类型：
--
作者：
Parpura,Vladimir
被称为NACAD，纳米制造的碳基探测器的探测器的制造过程中，描述。我用原子力和扫描电子显微镜技术表征了这个探测器的机械性能。该探测器具有光滑的表面（rms 2 - 4 nm），易于使用原子力显微镜尖端，这是分泌颗粒和囊泡生物学研究的必要功能，这些颗粒和囊泡储存递质并通过胞吐作用释放。NACAD不会损害沉积在检测器上的颗粒基质的弹性，因为它们显示出典型的杨氏模量和离子交换剂性质;二价组胺使它们收缩，而一价钠使它们膨胀。此外，NACADs的电化学性质允许安培测量从肥大细胞分离的完整分泌颗粒释放的5-羟色胺，在使用温和的去污剂处理去除其颗粒膜后。因此，这种检测器将有助于未来的研究单个分泌颗粒和囊泡及其不溶性基质。
The procedure for the fabrication of a detector termed NACAD, nanofabricated carbon-based detector, is described. I characterized the mechanical properties of this detector using atomic force and scanning electron microscopy techniques. This detector exhibits a smooth surface (rms 2−4 nm) and easy access for atomic force microscopy tips, necessary features for biological studies of secretory granules and vesicles, which store transmitters and release them via exocytosis. The NACAD does not impair the elastic properties of granular matrixes deposited onto a detector, as they show their typical Young's moduli and ion exchanger properties; divalent histamine shrinks them, while monovalent sodium causes their swelling. Additionally, the NACADs' electrochemical properties allowed amperometric measurements of serotonin released from intact secretory granules isolated from mast cells, after removal of their granular membranes using a mild detergent treatment. Thus, this detector will aid future studies of single secretory granules and vesicles and their insoluble matrixes.