TECHNIQUES FOR THE OPTIMUM RECOVERY OF COLD INJURED CAMPYLOBACTER-JEJUNI FROM MILK OR WATER

TECHNIQUES FOR THE OPTIMUM RECOVERY OF COLD INJURED CAMPYLOBACTER-JEJUNI FROM MILK OR WATER
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DOI:
10.1111/j.1365-2672.1986.tb04265.x
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发表时间:
1986-08-01
期刊:
JOURNAL OF APPLIED BACTERIOLOGY
影响因子:
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通讯作者:
HUMPHREY, TJ
HUMPHREY, TJ
中科院分区:
其他
文献类型:
--
作者:
HUMPHREY, TJ

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当肉汤接种细胞的空肠弯曲杆菌已被伤害的冷却有一个下降的可行的人口高达90%。它在43度时更大。37度。C和某些抗生素的存在下,在某些情况下,导致存活的种群低于随后生长的最低接种量。一种在37 ℃下在非选择性培养液中预富集的技术。在加入抗生素和在43 ℃温育之前,发现C显著减少了数量的下降,并提高了C的检测。生牛奶和水样品中的空肠。
When broth was inoculated with cells of Campylobacter jejuni that had been injured by chilling there was a fall in the viable population of up to 90%. It was greater at 43.degree. than 37.degree. C and in the presence of certain antibiotics and in some cases resulted in a surviving population that was below the minimum inoculum for subsequent growth. A technique of pre-enrichment in non-selective culture broth at 37.degree. C for 2 h before the addition of antibiotics and incubation at 43.degree. C was found to significantly reduce the fall in numbers and to improve the detection of C. jejuni in samples of raw milk and water.