NAM7 NUCLEAR GENE ENCODES A NOVEL MEMBER OF A FAMILY OF HELICASES WITH A ZN-LIGAND MOTIF AND IS INVOLVED IN MITOCHONDRIAL FUNCTIONS IN SACCHAROMYCES-CEREVISIAE
NAM7 NUCLEAR GENE ENCODES A NOVEL MEMBER OF A FAMILY OF HELICASES WITH A ZN-LIGAND MOTIF AND IS INVOLVED IN MITOCHONDRIAL FUNCTIONS IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0022-2836(92)90545-u
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发表时间：
1992-04-05
影响因子：
5.6
通讯作者：
SLONIMSKI, PP
中科院分区：
文献类型：
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作者：
ALTAMURA, N;GROUDINSKY, O;SLONIMSKI, PP
酵母核基因NAM7是在7.7kb(1kb=103个碱基或碱基对)的基因组片段中分离出来的，具有抑制线粒体RNA剪接缺陷内含子突变的能力。我们已经鉴定并测序了与NAM7基因相对应的插入物上的区域。一个长的开放阅读框已经被发现，它可以编码一个971个氨基酸的蛋白质。将NAM7推测的蛋白质与数据库进行比较，没有发现与已知蛋白质有任何强烈的相似性。然而，NAM7蛋白包含几个典型的蛋白质与核酸相互作用的基序：(1)一个解旋酶超家族的五个基序以相同的顺序和相似的距离出现；(2)N端部分具有潜在的锌配体结构，属于Cx超家族。NAM7基因染色体拷贝的缺失会导致呼吸生长的部分损害，这种损害在低温下尤为明显。Southern杂交分析表明，从缺失的anam7：URA3菌株中提取的DNA中存在第二个基因，该基因的序列与NAM7基因的序列有关，并可能参与相同的过程。
The yeast nuclear geneNAM7was previously isolated within a genomic fragment of 7.7 kb (1 kb = 103bases or base-pairs), having the ability to suppress mitochondrial intronic mutations defective in RNA splicing. We have identified and sequenced the region on the insert corresponding to theNAM7gene. A long open reading frame has been revealed which could code for a protein of 971 amino acids. Comparison of the NAM7 putative protein with data libraries did not reveal any strong similarity with known proteins. However, the NAM7 protein contains several motifs typical for proteins interacting with nucleic acids: (1) five motifs diagnostic for a superfamily of helicases appear in the same order and with similar distances; (2) the N-terminal portion possesses potential Zn-ligand structures belonging to the Cxsuperfamily. Deletion of the chromosomal copy ofNAM7gene leads to a partial impairment in respiratory growth that is particularly striking at low temperature. Southern blot analysis of DNA extracted from anam7:URA3deleted strain revealed the presence of a second gene whose sequence is related to that of theNAM7gene and which could participate in the same process.