Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
Longitudinal Analyses of Gut Mucosal Microbiotas in Ulcerative Colitis in Relation to Patient Age and Disease Severity and Duration
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DOI：
10.1128/jcm.02574-12
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发表时间：
2013-03-01
影响因子：
9.4
通讯作者：
Macfarlane, George T.
中科院分区：
文献类型：
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作者：
Fite, Alemu;Macfarlane, Sandra;Macfarlane, George T.
属于正常结肠微生物群的细菌与溃疡性结肠炎（UC）的病因学相关。虽然有几种粘膜物种与疾病过程有关，但所涉及的生物体和机制尚不清楚。本研究的目的是表征随时间推移的粘膜生物膜群落，并确定这些细菌与患者年龄、疾病严重程度和持续时间的关系。多个直肠活检标本取自33例活动性UC患者，为期1年。采用实时PCR定量UC患者和18名非炎症性肠病对照者的粘膜细菌，并通过线性回归分析评估疾病严重程度指标与细菌定植之间的关系。在UC粘膜和对照组中检测到细菌种群的显著差异，其在研究期间变化。高临床活动指数（CAI）和乙状结肠镜检查评分（SS）与肠杆菌、产气荚膜梭菌、E型产气荚膜梭菌和粪肠球菌相关，而丁酸梭菌、白色瘤胃球菌和直肠真杆菌则相反。乳酸杆菌和双歧杆菌的数量与低CAI有关。只有E.直肠梭状芽胞杆菌和梭状芽胞杆菌具有高度的年龄依赖性。这些发现表明，在UC中发生粘膜细菌种群的纵向变化，并且细菌群落结构与疾病严重程度相关。
Bacteria belonging to the normal colonic microbiota are associated with the etiology of ulcerative colitis (UC). Although several mucosal species have been implicated in the disease process, the organisms and mechanisms involved are unknown. The aim of this investigation was to characterize mucosal biofilm communities over time and to determine the relationship of these bacteria to patient age and disease severity and duration. Multiple rectal biopsy specimens were taken from 33 patients with active UC over a period of 1 year. Real-time PCR was used to quantify mucosal bacteria in UC patients compared to 18 noninflammatory bowel disease controls, and the relationship between indicators of disease severity and bacterial colonization was evaluated by linear regression analysis. Significant differences were detected in bacterial populations on the UC mucosa and in the control group, which varied over the study period. High clinical activity indices (CAI) and sigmoidoscopy scores (SS) were associated with enterobacteria, desulfovibrios, type E Clostridium perfringens, and Enterococcus faecalis, whereas the reverse was true for Clostridium butyricum, Ruminococcus albus, and Eubacterium rectale. Lactobacillus and bifidobacterium numbers were linked with low CAI. Only E. rectale and Clostridium clostridioforme had a high age dependence. These findings demonstrated that longitudinal variations in mucosal bacterial populations occur in UC and that bacterial community structure is related to disease severity.